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Team:Heidelberg/Templates/DelH week23
From 2013.igem.org
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Expected band: 663 bp | Expected band: 663 bp | ||
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| - | [[|200px|thumb|right|'''Fig. | + | [[|200px|thumb|right|'''Fig.23.1''' Colony PCR of Gibson assembled DelH-BB without mRFP 23 colonies]] |
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Revision as of 13:28, 22 October 2013
Contents |
30-09 - 06-10-13
Generation of DelH Plasmid pHM04 15-09
Colony-PCR Conditions CP.W23.A
| Template | 23x 1 picked colony, positive control |
| Expected length [bp] | 663 |
| Named | 1A - 12H |
| Primer fw 10 µM | 100x 2 µl VF2 |
| Primer rev 10 µM | 100x 2 µl DN07 |
| iTaq Polymerase (2x) | 100x 10 µl |
| ddH2O | 96x 6 µl |
| Cycles | Temperature DelH [°C] | Time [s] |
|---|---|---|
| 1 | 95 | 120 |
| 12 | 95 | 30 |
| 68 (touchdown -0.5°C) | 30 | |
| 72 | 45 | |
| 18 | 95 | 30 |
| 65 | 30 | |
| 72 | 45 | |
| 1 | 12 | inf |
Result
Expected band: 663 bp
[[|200px|thumb|right|Fig.23.1 Colony PCR of Gibson assembled DelH-BB without mRFP 23 colonies]]
