Team:Heidelberg/Templates/Del week10 OP

From 2013.igem.org

Revision as of 14:07, 30 September 2013 by Nils.kurzawa (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

04-07-2013

Amplification from FS_12 to FS_13; 2.7 kb

Gel eletrophoresis of PCRs of DelF-G, DelO-P and DelL


Reaction
what µl
D. acidovorans DSM-39 1
FS_12: (1/10) 1
FS_13: (1/10) 1
Phusion Master Mix 10
DMSO 1
dd H2O 6
Conditions
Biorad MyCycler
Cycles temperature [°C] Time [s]
1 98 10
30 98 1
72 40
1 72 420
1 4 inf

Results:

  • Amplification of DelOP did not work
  • Amplification will be repeated to exclude that pipetting errors were the reason for the failure