Team:HokkaidoU Japan/Notebook/RBS SD2,4,6-Reconstruction

From 2013.igem.org

Revision as of 16:38, 27 September 2013 by TaKeZo (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Maestro E.coli

Notebook

SD2,4,6 Reconstruction

  • Start

  • PCR

    R0040-SD8-LacZα-B0015 100up SD(6/4/2) mutation revers
  • Gel extraction

    R0040-SD(6/4/2)
  • Digestion

    Cut R0040-SD(6/4/2) with EcoRI and SpeI (10×H buffer)
  • Ethanol precipitation

    R0040-SD(6/4/2)
  • Ligation

    Ligate R0040-SD(6/4/2) with LacZα-B0015
  • Transformation

    R0040-SD(6/4/2)-LacZα-B0015
    each of 5µL DNA to DH5α
  • Sequencing

    R0040-SD(6/4/2)-LacZα-B0015
  • Complete!