Team:Hong Kong CUHK

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=Project Description=
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The goal of our project is to develop a method using E.coli to degrade polycyclic aromatic hydrocarbons (PAHs) into other less toxic chemicals. PAHs are notorious for their damage on the environment and human health. These compounds can be found anywhere, from second-hand smoke to cooking fume. The wide spread and toxicity of PAHs indeed arouse our concern and interest to tackle the issue.
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We propose a pathway of PAH degradation, which is carried out by a combination of enzyme laccase and catechol 1, 2-dioxygenase. The final product is a kind of simple carboxylic acid, which is less harmful to human body. Since quinones are generated in the beginning of the metabolism, we use quinone sensing and response repressor (QsrR) to control the degradation process.
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Another great highlight of our project is Voltage Switch. In order to increase the efficiency of PAHs degradation pathway, we design a novel switch triggered by electricity. The switch includes differently combined protein domains that regulate the distance between enzymes. It allows fast control of concentration of reaction mixture, thus achieving fast response by electricity. Apart from using Voltage Switch, we also try to link the two enzymes together as a reaction complex by Protein Scaffolding, the project from SJTU 2012 iGEM team.
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  <h1>Abstract</h1>
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  <p2>To rapidly regulate biological process, we designed a novel transmembrane protein called Voltage Switch (VS), which is a fusion protein utilizing the voltage sensing domain from potassium ion channels. Triggered by a change of potential across the inner membrane, VS can bring targeting enzymes into proximity, thus allowing an instant control of enzymatic reaction...</p2>
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  <h1>Human Practice</h1>
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  <p2>Interest and mindset formation should start at young  age for permanence and  continuity. High school and university students are  great audiences  to be  approached to break the blurred paradigm of synthetic  biology, which term is rarely  known by the general public...</p2>
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  <h1>Voltage Switch</h1>
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  <p2>Voltage Switch is a response system triggered by a change of potential across the bacterial inner membrane. Components of this system include the transmembrane voltage sensor peptide originated from potassium ion channels, polyproline linker in the inner membrane, glycine serine linker, PDZ domain and PDZ ligand in the periplasm, and glycine serine linker and effectors in the cytosol...</p2>
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  <h1>PAH Degradation</h1>
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  <p2>Our project initiated from dealing with toxic chemicals found in second-hand smoke (or environmental  tobacco smoke, ETS) and cooking fume. After carefully examining the composition  of pollutes, we noticed that there was one group of highly toxic...</p2>
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  Address: Room 184, Science Centre, CUHK <br/>
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  Email: kingchan@cuhk.edu.hk  Tel: (852)-39434420  Fax: (852)-26037246
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Latest revision as of 02:10, 29 October 2013

iGEM CUHK

Retrieved from "http://2013.igem.org/Team:Hong_Kong_CUHK"