Team:Hong Kong HKU

From 2013.igem.org

(Difference between revisions)
Line 1: Line 1:
<html>
<html>
-
      <meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
+
{{:Team:Hong_Kong_HKU/templates/header_v2}}
-
      <meta name="title" content="HKU iGEM 2013" />
+
{{:Team:Hong_Kong_HKU/templates/floatingmenu/project}}
-
      <meta name="keywords" content="iGEM, HKU, Synthetic Biology, PPK" />
+
-
      <meta name="description" content="HKU's IGEM Team. We aim to isolate phosphate using a labelled microcompartment.">
+
-
    <style type="text/css">
+
<html>
-
    /* Compulsory IDs ***********************************************************/
+
-
      #catlinks {
+
-
      border:none;
+
-
      background: #ffffff;
+
-
      }
+
-
      #footer-box{
+
-
      border:none;
+
-
      }
+
-
      #top-section {
+
-
      background-color: #ffffff;
+
-
      border: none;
+
-
      height:20px;
+
-
      }
+
-
      #p-logo {
+
-
      display: none;
+
-
      }
+
-
      #search-controls {
+
-
      display: none;
+
-
      }
+
-
      .firstHeading {
+
-
        display: none;
+
-
      }
+
-
     
+
-
      #content {
+
-
      position: relative;
+
-
      width: 975px;
+
-
      margin: 0 auto;
+
-
      padding:0;
+
-
      background: #ffffff;
+
-
      color: black;
+
-
      border: 1px solid #d9d9d9;
+
-
      line-height: 1.5em;
+
-
      z-index: 2;
+
-
      }
+
-
      body {
+
-
      background-color:#ffffff;
+
-
      }
+
-
      .right-menu li a, .right-menu li a:hover {  /*reserved*/
+
-
      color: #3c6b27;
+
-
      background-color: #ffffff;
+
-
      }
+
-
      /*#menucontainer {
+
-
      overflow:visible;
+
-
      position:relative;
+
-
      z-index:3;
+
-
      }*/
+
-
      #contentSub {
+
-
      margin: 0 0 0 0;
+
-
      } 
+
-
 
+
-
    </style>
+
<body>
<body>
-
<style type="text/css">
+
<div id="wrapper">  
-
 
+
<div id="content1">  
-
</style>
+
-
<div id="frontpage">
+
<br>
<br>
-
<br>
 
-
<img src="https://static.igem.org/mediawiki/igem.org/3/3d/Hkudothingsonamicroscalelogo.jpg" width="960" height="540">
 
-
 
-
<p style="color: #000; font-size: 16px; font-family: Baskerville, 'Palatino Linotype', Palatino, 'Century Schoolbook L', 'Times New Roman', serif; text-align: left;">
 
-
 
-
<br>
 
-
<b>Bacterial Microcompartments (BMCs)</b> are closed polyhedral macromolecular complexes with a diameter of 100-150 nm, enclosing enzymes and cofactors for various metabolism reactions. One of the example of such endogenous capsid micro-reactors is the <b><u>E</u>thanolamine <u>ut</u>ilization (Eut)</b> BMC from <i>Salmonella enterica</i> spp.
 
-
<br><br>
 
-
In <i>Salmonella enterica</i> spp., 5 genes (Eut S, M, N, L, K) encodes thousands of copies of shell proteins to form a heterogenous MCP shell. Such empty, recombinant Eut BMCs has been successfully cloned and expressed in <i>Escherichia coli</i>. In addition, a localization signal has been identified which can be fused into the N-terminus of unnaturally-encapsulated enzymes or proteins to facilitate their localization into the microcompartment
 
-
<br><br>
 
-
Inspired by these studies, we speculated that this Eut BMC can become a versatile tool if we can:
 
-
<br>(a) modify the exterior surface to confer various novel physical, chemical, and biochemical properties to the whole capsule;
 
-
<br>(b) localize special enzyme into the BMC for specfic functionalization;
 
-
<br>(c) store or recycle useful and harmful molecules into the BMC
 
-
<br><br>
 
-
<!---someone fix the alignment please, also note that the bolding and codings are not Wiki-styled, there must be some fundamental coding problems occuring here--->
 
-
<img src="https://static.igem.org/mediawiki/2013/8/87/BMC_with_His_tag_Fig.1_00000.jpg" width="240" height="216">
 
-
<img src="https://static.igem.org/mediawiki/2013/5/52/PPK1_in_action_design_Fig.2_00000.jpg" width="240" height="216">
 
-
<br><br>
 
-
As a proof of concept, we displayed His tag on the surface of the Eut BMCs (Fig. 1), and at the same time, we fused the localization signal to polyphosphate kinase 1 from <i>Tannerella forsythia</i> (Fig. 2). After cloning both of the above constructs into <i>Escherichia coli</i>, we hope to demonstrate the one application of such integration by recycling phosphates from the polluted waters aided by the activity of polyphosphate kinase and encapsulating characteristics of Eut BMC.
 
-
<br><br>
 
<table width="960px" cellpadding="0px">
<table width="960px" cellpadding="0px">
<tr>
<tr>
Line 103: Line 27:
</td>
</td>
</tr>
</tr>
-
 
-
 
</table>
</table>
-
 
+
<br><br>
-
</p>
+
</div>
-
<p style="font-size:36px">
+
</div>
<a href="https://2013.igem.org/Team:Hong_Kong_HKU/Safety">Click here to go to the safety page</a>
<a href="https://2013.igem.org/Team:Hong_Kong_HKU/Safety">Click here to go to the safety page</a>
-
</p>
 
-
</div>
 
-
 
</body>
</body>
-
 
-
<STYLE TYPE="text/css">
 
-
#frontpage{
 
-
text-align:center;
 
-
}
 
-
</STYLE>
 
</html>
</html>
 +
{{:Team:Hong_Kong_HKU/templates/footer}}

Revision as of 16:14, 24 September 2013

{{:Team:Hong_Kong_HKU/templates/header_v2}} {{:Team:Hong_Kong_HKU/templates/floatingmenu/project}}

Click here to go to the safety page