Team:Hong Kong HKU

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<b>Bacterial Microcompartments (BMCs)</b> are closed polyhedral macromolecular complexes with a diameter of 100-150 nm, enclosing enzymes and cofactors for various metabolism reactions. One of the example of such endogenous capsid micro-reactors is the <b><u>E</u>thanolamine <u>ut</u>ilization (Eut)</b> BMC from <i>Salmonella enterica</i> spp.
 
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In <i>Salmonella enterica</i> spp., 5 genes (Eut S, M, N, L, K) encodes thousands of copies of shell proteins to form a heterogenous MCP shell. Such empty, recombinant Eut BMCs has been successfully cloned and expressed in <i>Escherichia coli</i>. In addition, a localization signal has been identified which can be fused into the N-terminus of unnaturally-encapsulated enzymes or proteins to facilitate their localization into the microcompartment
 
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Inspired by these studies, we speculated that this Eut BMC can become a versatile tool if we can:
 
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<br>(a) modify the exterior surface to confer various novel physical, chemical, and biochemical properties to the whole capsule;
 
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<br>(b) localize special enzyme into the BMC for specfic functionalization;
 
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<br>(c) store or recycle useful and harmful molecules into the BMC
 
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<!---someone fix the alignment please, also note that the bolding and codings are not Wiki-styled, there must be some fundamental coding problems occuring here--->
 
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<img src="https://static.igem.org/mediawiki/2013/8/87/BMC_with_His_tag_Fig.1_00000.jpg" width="240" height="216">
 
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As a proof of concept, we displayed His tag on the surface of the Eut BMCs (Fig. 1), and at the same time, we fused the localization signal to polyphosphate kinase 1 from <i>Tannerella forsythia</i> (Fig. 2). After cloning both of the above constructs into <i>Escherichia coli</i>, we hope to demonstrate the one application of such integration by recycling phosphates from the polluted waters aided by the activity of polyphosphate kinase and encapsulating characteristics of Eut BMC.
 
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<a href="https://2013.igem.org/Team:Hong_Kong_HKU/at_a_glance" target="_new">  
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<a href="https://2013.igem.org/Team:Hong_Kong_HKU/Safety">Click here to go to the safety page</a>
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Latest revision as of 13:08, 11 October 2014

mhvnh h



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