"
Team:Hong Kong HKUST/notebook/mod2
From 2013.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
.head, li, h2 { margin-bottom:15px;z-index:-11; } | .head, li, h2 { margin-bottom:15px;z-index:-11; } | ||
.head { display:block; background:#35BB91;border-radius:500px;margin-bottom:5px;align:center;width:90%;z-index:-3; } | .head { display:block; background:#35BB91;border-radius:500px;margin-bottom:5px;align:center;width:90%;z-index:-3; } | ||
| - | .content { display:none; background:#8FF5D5;border-radius: | + | .content { display:none; background:#8FF5D5;border-radius:100px;margin-bottom:5px;align:center;width:90%;z-index:-3;padding-left:100px;padding-right:100px;} |
li { position:relative;overflow:hidden; } | li { position:relative;overflow:hidden; } | ||
</style> | </style> | ||
| Line 300: | Line 300: | ||
<div> | <div> | ||
<a href='#' class='head'>Week 4</a> | <a href='#' class='head'>Week 4</a> | ||
| - | <div class='content' align="left">Wet lab<br> | + | <div class='content' align="left"> |
| - | · Inoculation of pBlueScript KS(+) for training<br> | + | <br>June 24 <br> |
| - | · Autoclave basic materials< | + | Wet lab <br> |
| - | · Preparing LB<br> | + | · Inoculation of pBlueScript KS(+) for training <br> |
| - | · LB-Ampicillin plates poured<br> | + | · Autoclave basic materials <br> |
| - | Dry lab<br> | + | · Preparing LB <br> |
| - | + | · LB-Ampicillin plates poured | |
| + | Dry lab <br> | ||
| + | Protocols review <br> | ||
| + | <br> | ||
| + | June 25 <br> | ||
| + | Wet lab <br> | ||
| + | · Plasmid extraction of pBlueScript KS(+) <br> | ||
| + | · LB-Chloramphenicol plates poured <br> | ||
| + | Dry lab <br> | ||
| + | · Primers design for FABP1, PPARa, GRP78 <br> | ||
| + | · Informal meeting <br> | ||
| + | <br> | ||
| + | June 26 <br> | ||
| + | Wet lab <br> | ||
| + | · Extraction of gDNA from HepG2 Cells for FABP1 and PPARa <br> | ||
| + | · Transformation of pEGFP-N1 <br> | ||
| + | · Transformation of BBa_K817002 (pFadBA) <br> | ||
| + | <br> | ||
| + | June 27 <br> | ||
| + | Wet lab <br> | ||
| + | · Inoculation of pEGFP-N1 for plasmid extraction <br> | ||
| + | · Inoculation of BBa_K817002 (pFadBA) for plasmid extraction <br> | ||
| + | <br> | ||
| + | June 28 <br> | ||
| + | Wet lab <br> | ||
| + | · Extraction of pEGFP-N1 plasmids by miniprep <br> | ||
| + | · Extraction of BBa_K817002 (pFadBA) <br> | ||
| + | · Restriction of pEGFP-N1 Restriction digestion by Ase1 and bamH1 for PPARa; Ase1 and Xho1 for GRP78, Xba1 and BamH1 for FABP1, Pst1 and Not1; followed by gel check, extraction and purification <br> | ||
| + | · Restriction of c(pFadBA) by EcoR1 and Pst1 <br> | ||
| + | · Ran 0.8% gel for pEGFP-N1 <br> | ||
| + | · Ran 2% gel for BBa_K817002 (pFadBA) <br> | ||
| + | <br> | ||
| + | June 30 <br> | ||
| + | Dry lab <br> | ||
| + | Experiment planning and protocols revision for next week work<br></br> | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 14:35, 19 September 2013
June 2013
Week 4
June 24
Wet lab
· Inoculation of pBlueScript KS(+) for training
· Autoclave basic materials
· Preparing LB
· LB-Ampicillin plates poured Dry lab
Protocols review
June 25
Wet lab
· Plasmid extraction of pBlueScript KS(+)
· LB-Chloramphenicol plates poured
Dry lab
· Primers design for FABP1, PPARa, GRP78
· Informal meeting
June 26
Wet lab
· Extraction of gDNA from HepG2 Cells for FABP1 and PPARa
· Transformation of pEGFP-N1
· Transformation of BBa_K817002 (pFadBA)
June 27
Wet lab
· Inoculation of pEGFP-N1 for plasmid extraction
· Inoculation of BBa_K817002 (pFadBA) for plasmid extraction
June 28
Wet lab
· Extraction of pEGFP-N1 plasmids by miniprep
· Extraction of BBa_K817002 (pFadBA)
· Restriction of pEGFP-N1 Restriction digestion by Ase1 and bamH1 for PPARa; Ase1 and Xho1 for GRP78, Xba1 and BamH1 for FABP1, Pst1 and Not1; followed by gel check, extraction and purification
· Restriction of c(pFadBA) by EcoR1 and Pst1
· Ran 0.8% gel for pEGFP-N1
· Ran 2% gel for BBa_K817002 (pFadBA)
June 30
Dry lab
Experiment planning and protocols revision for next week work
June 24
Wet lab
· Inoculation of pBlueScript KS(+) for training
· Autoclave basic materials
· Preparing LB
· LB-Ampicillin plates poured Dry lab
Protocols review
June 25
Wet lab
· Plasmid extraction of pBlueScript KS(+)
· LB-Chloramphenicol plates poured
Dry lab
· Primers design for FABP1, PPARa, GRP78
· Informal meeting
June 26
Wet lab
· Extraction of gDNA from HepG2 Cells for FABP1 and PPARa
· Transformation of pEGFP-N1
· Transformation of BBa_K817002 (pFadBA)
June 27
Wet lab
· Inoculation of pEGFP-N1 for plasmid extraction
· Inoculation of BBa_K817002 (pFadBA) for plasmid extraction
June 28
Wet lab
· Extraction of pEGFP-N1 plasmids by miniprep
· Extraction of BBa_K817002 (pFadBA)
· Restriction of pEGFP-N1 Restriction digestion by Ase1 and bamH1 for PPARa; Ase1 and Xho1 for GRP78, Xba1 and BamH1 for FABP1, Pst1 and Not1; followed by gel check, extraction and purification
· Restriction of c(pFadBA) by EcoR1 and Pst1
· Ran 0.8% gel for pEGFP-N1
· Ran 2% gel for BBa_K817002 (pFadBA)
June 30
Dry lab
Experiment planning and protocols revision for next week work
