Team:INSA Toulouse/contenu/project/biological construction/carry

From 2013.igem.org

(Difference between revisions)
(Created page with "{{:Team:INSA_Toulouse/template/header}} {{:Team:INSA_Toulouse/template/sidebar}} <!--Contenu*/ /**********/--> <html> <!--- open Sans : fonnt Google: --> <link href='htt...")
 
(11 intermediate revisions not shown)
Line 70: Line 70:
<div class="maincontent" style="width: 720px; margin: 25px 0 25px 0; float: right;">
<div class="maincontent" style="width: 720px; margin: 25px 0 25px 0; float: right;">
-
  <h1 class="title1">Project</h1>
 
-
   <h2 class="title2">Abstract</h2>
+
   <h1 class="title1">Biological Modules: Carry</h1>
-
  <p class="texte">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut et dolor turpis. Suspendisse interdum, dolor eu  ultricies dignissim, arcu sapien placerat quam, ut bibendum lorem nibh id magna. Nunc condimentum lectus at diam tempus sodales. Nullam scelerisque auctor tellus, nec auctor ante elementum sit amet. Aenean venenatis velit eget porttitor laoreet. Aliquam aliquam libero in ante imperdiet vehicula. Donec nibh urna, tincidunt quis condimentum at, tempus sed sapien. Nunc elit dui, dignissim tincidunt mi non, ultrices ultricies erat.</p>
+
  <h2 class="title2"></h2>
-
 
+
-
  <img src="https://static.igem.org/mediawiki/2013/b/b7/Insa-toulouse2013-phototestpage.jpg" class="imgcontent" />
+
-
   <p class="texteleft"><span class="spantitle">lorem ipsum</span></br>
+
   <p class="texte">For the carry we thought of a molecule that could transmit a message from one colony to the other.<br>
-
<br>Mauris ac suscipit erat, sit amet blandit lectus. Fusce placerat, lectus at suscipit viverra, lorem lorem mattis turpis, id pharetra velit nunc vitae velit. Etiam ultrices aliquam ligula, sed ultrices nibh vulputate non. Ut dapibus arcu luctus, suscipit urna et, imperdiet magna. Curabitur tristique sed diam non elementum. Sed lectus urna, consequat quis porta eu, ultrices in nunc. Aenean vitae elit neque.<p>
+
  The communication between cells in Quorum Sensing is a good illustration of the diffusion of a signal between unicellular organisms. We resorted to the system present in <i>Vibrio fischeri</i> using N-Acyl homoserine lactone (AHL). </p>
-
  <p class="texteright"><span class="spantitle">lorem ipsum</span></br>
+
      <p class="texteleft"> <i>General principle</i>
-
<br>Mauris ac suscipit erat, sit amet blandit lectus. Fusce placerat, lectus at suscipit viverra, lorem lorem mattis turpis, id pharetra velit nunc vitae velit. Etiam ultrices aliquam ligula, sed ultrices nibh vulputate non. Ut dapibus arcu luctus, suscipit urna et, imperdiet magna. Curabitur tristique sed diam non elementum. Sed lectus urna, consequat quis porta eu, ultrices in nunc. Aenean vitae elit neque.</p>
+
<br>AHL is the product of the LuxI gene. On a basic <i>E.calculus colony</i>, this one was placed under the control of promoters belonging to the two AND gates. AHL will be produced when at least one of the AND gates is activated. Then AHL will be transmitted to the following colony by diffusion into LBagar via the Quorum Sensing. It becomes a third input signal (in addition of blue/red lights) that will be taken into consideration for the calculation of the next bit: this is the carry system of our full adder.
-
 
+
  </p>
-
<div class="clear"></div>
+
-
 
+
-
  <div class="list">
+
      
      
-
    <ul class="arrow">
+
     <p class="texteright"> <i>Carry module characterization </i>
-
      <li>Quisque at massa ipsum</li>
+
<br> AHL diffusion into agar medium was characterized thanks to the Chromobacterium violaceum strain. A purple coloration is visible when C. violaceum is in contact with AHL.  
-
      <li>Maecenas a lorem augue, egestas </li>
+
  </p>
-
      <li>Cras vitae felis at lacus ele </li>
+
-
      <li>Etiam auctor diam pellentesque </li>
+
-
      <li>Nulla ac massa at dolor </li>
+
-
    </ul>
+
-
 
+
-
  </div>
+
-
 
+
-
  <div class="list">
+
-
      
+
-
    <ul class="circlearrow">
+
-
      <li>Quisque at massa ipsum</li>
+
-
      <li>Maecenas a lorem augue, egestas </li>
+
-
      <li>Cras vitae felis at lacus ele </li>
+
-
      <li>Etiam auctor diam pellentesque </li>
+
-
      <li>Nulla ac massa at dolor </li>
+
-
    </ul>
+
-
 
+
-
  </div>
+
-
 
+
-
  <div class="list">
+
-
   
+
-
    <ul class="arrow">
+
-
      <li>Quisque at massa ipsum</li>
+
-
      <li>Maecenas a lorem augue, egestas </li>
+
-
      <li>Cras vitae felis at lacus ele </li>
+
-
      <li>Etiam auctor diam pellentesque </li>
+
-
      <li>Nulla ac massa at dolor </li>
+
-
    </ul>
+
-
 
+
-
  </div>
+
-
 
+
-
<div class="clear"></div>
+
-
 
+
-
  <h3 class="title3">TITRE 3 lorem ipsum</h3>
+
-
 
+
-
  <p class="texte">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut et dolor turpis. Suspendisse interdum, dolor eu  ultricies dignissim, arcu sapien placerat quam, ut bibendum lorem nibh id magna. Nunc condimentum lectus at diam tempus sodales. Nullam scelerisque auctor tellus, nec auctor ante elementum sit amet. Aenean venenatis velit eget porttitor laoreet. Aliquam aliquam libero in ante imperdiet vehicula. Donec nibh urna, tincidunt quis condimentum at, tempus sed sapien. Nunc elit dui, dignissim tincidunt mi non, ultrices ultricies erat.</p>
+
-
 
+
-
  <table class="tablecontent">
+
-
 
+
-
<tr style="background-color:#20a8da; height:50px; color:#ffffff;" >
+
-
<td style="border-bottom:4px solid #e5e6e6; border-top-left-radius:9px;">TR  Title</td>
+
-
<td style="border-bottom:4px solid #e5e6e6;">TR  Title</td>
+
-
<td style="border-bottom:4px solid #e5e6e6; border-top-right-radius:9px;">TR  Title</td>
+
-
</tr>
+
-
<tr>
+
-
<td style="border-right:1px solid #e5e6e6;">Quisque</td>
+
-
<td style="border-right:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
-
<td>Maecenas a lorem augue</td>
+
-
</tr>
+
-
<tr style="border-top:1px solid #e5e6e6">
+
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Quisque</td>
+
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
-
<td style="border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
-
</tr>
+
-
<tr>
+
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Quisque</td>
+
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
-
<td style="border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
-
</tr>
+
-
<tr>
+
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Quisque</td>
+
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
-
<td style="border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
-
</tr>
+
-
</table>
+
-
 
+

Latest revision as of 17:50, 4 October 2013

logo


Biological Modules: Carry

For the carry we thought of a molecule that could transmit a message from one colony to the other.
The communication between cells in Quorum Sensing is a good illustration of the diffusion of a signal between unicellular organisms. We resorted to the system present in Vibrio fischeri using N-Acyl homoserine lactone (AHL).

General principle
AHL is the product of the LuxI gene. On a basic E.calculus colony, this one was placed under the control of promoters belonging to the two AND gates. AHL will be produced when at least one of the AND gates is activated. Then AHL will be transmitted to the following colony by diffusion into LBagar via the Quorum Sensing. It becomes a third input signal (in addition of blue/red lights) that will be taken into consideration for the calculation of the next bit: this is the carry system of our full adder.

Carry module characterization
AHL diffusion into agar medium was characterized thanks to the Chromobacterium violaceum strain. A purple coloration is visible when C. violaceum is in contact with AHL.