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Team:Imperial College/3HB assay
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<h1 class="clear">3HB assay</h1> | <h1 class="clear">3HB assay</h1> | ||
| - | <p>This experiment completes the cycle of PHB bioplastic recycling. We measured the absorbance at 445, 450 and 455 | + | <p><b>This experiment completes the cycle of PHB bioplastic recycling. </b></p> |
| - | + | <p>We prepared serveral dilutions of the samples and measured the absorbance at 445, 450 and 455. | |
| + | </p> | ||
| + | https://static.igem.org/mediawiki/parts/1/1a/Collected_data_for_3HB_assay.JPG | ||
| + | <p>We corrected the absorbance values busubstracting the 0 standard.</p> | ||
https://static.igem.org/mediawiki/parts/5/55/Data_collection_3HB_assay2.JPG | https://static.igem.org/mediawiki/parts/5/55/Data_collection_3HB_assay2.JPG | ||
| - | + | <We plotted the standard curve and used the below formula to calculate the 3HB concentrations in our samples</p> | |
https://static.igem.org/mediawiki/parts/e/e3/Formula_for_calculation.JPG | https://static.igem.org/mediawiki/parts/e/e3/Formula_for_calculation.JPG | ||
Revision as of 21:18, 2 October 2013
3HB assay
This experiment completes the cycle of PHB bioplastic recycling.
We prepared serveral dilutions of the samples and measured the absorbance at 445, 450 and 455.
We corrected the absorbance values busubstracting the 0 standard.
<We plotted the standard curve and used the below formula to calculate the 3HB concentrations in our samples</p>
