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Team:Imperial College/3HB assay
From 2013.igem.org
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| - | <h1 class="clear">3HB assay</h1> | + | <h1 class="clear">3HB assay #1</h1> |
<p><b>This experiment completes the cycle of PHB bioplastic recycling. </b></p> | <p><b>This experiment completes the cycle of PHB bioplastic recycling. </b></p> | ||
<p>Please see [https://2013.igem.org/Team:Imperial_College/Protocols#3HB_assay 3HB protocol] for sample preparation and the way we adapted the kit for our purposes.</p> | <p>Please see [https://2013.igem.org/Team:Imperial_College/Protocols#3HB_assay 3HB protocol] for sample preparation and the way we adapted the kit for our purposes.</p> | ||
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<p>We have taken the dynamic range of the assay into account [https://www.caymanchem.com/pdfs/700190.pdf (according to protocol)] and therefore excluded readings where the concentration of 3HB in the well was above 1 mM because these are not accurate. </p> | <p>We have taken the dynamic range of the assay into account [https://www.caymanchem.com/pdfs/700190.pdf (according to protocol)] and therefore excluded readings where the concentration of 3HB in the well was above 1 mM because these are not accurate. </p> | ||
https://static.igem.org/mediawiki/parts/d/d1/3HB_from_PHB_from_waste.jpg | https://static.igem.org/mediawiki/parts/d/d1/3HB_from_PHB_from_waste.jpg | ||
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| + | <h1 class="clear">3HB assay #2</h1> | ||
| + | https://2013.igem.org/File:Full_annotated_diagram.jpg | ||
{{:Team:Imperial_College/Templates:footer}} | {{:Team:Imperial_College/Templates:footer}} | ||
Revision as of 15:44, 27 October 2013
3HB assay #1
This experiment completes the cycle of PHB bioplastic recycling.
Please see 3HB protocol for sample preparation and the way we adapted the kit for our purposes.
The well setup was as shown below:
We prepared serveral dilutions of the samples and measured the absorbance at 445, 450 and 455.
We corrected the absorbance values busubstracting the 0 standard.
We plotted the standard curve and used the below formula to calculate the 3HB concentrations in our samples.
We have taken the dynamic range of the assay into account (according to protocol) and therefore excluded readings where the concentration of 3HB in the well was above 1 mM because these are not accurate.
3HB assay #2
https://2013.igem.org/File:Full_annotated_diagram.jpg
