Team:KU Leuven/Project

From 2013.igem.org

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The current solution usually requires huge amounts of insecticides, damaging ecosystems in several ways. First, the indiscriminate weakening of insects means that beneficial insects (pollinators, ...) are also affected. Second, insecticides affect vertebrates, including humans, damaging our health. Third, insecticide residues (catabolites) accumulate in each food-chain. The highest organism, be it humans or for example predatory birds, will end up with potentially high levels of residues and their negative effects.<br/>
The current solution usually requires huge amounts of insecticides, damaging ecosystems in several ways. First, the indiscriminate weakening of insects means that beneficial insects (pollinators, ...) are also affected. Second, insecticides affect vertebrates, including humans, damaging our health. Third, insecticide residues (catabolites) accumulate in each food-chain. The highest organism, be it humans or for example predatory birds, will end up with potentially high levels of residues and their negative effects.<br/>
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We aim to offer an <b>effective and sustainable alternative for insecticides.</b> Our modified <I>E. coli</I>, the <B>BanAphids</B> (meaning ‘to ban aphids’ as well as provide plants with ‘benefits’), would produce the aphid’s own alarm pheromone, <b>E-β-farnesene (EBF)</b>, to repel them off the plant. On top of that we want to attract aphid predators such as the ladybug via the production of <b>Methyl Salicylate (MeS)</b>, a phytohormone, by our BanAphids. This way we ensure aphids are thoroughly removed from the plant. More about this can be found <a href="https://2013.igem.org/Team:KU_Leuven/HoneydewSystem"> here.</a> <br/>
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We aim to offer an <b>effective and sustainable alternative for insecticides.</b> Our modified <I>E. coli</I>, the <B>BanAphids</B> (meaning ‘to ban aphids’ as well as provide plants with ‘benefits’), would produce the aphid’s own alarm pheromone, <b>E-β-farnesene (EBF)</b>, to repel them off the plant. On top of that we want to attract aphid predators such as the ladybug via the production of <b>Methyl Salicylate (MeS)</b>, a phytohormone, by our BanAphids. This way we ensure aphids are thoroughly removed from the plant.
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We devised <b>two different systems</b> that might use these molecules. We did this because of response we received from both private end users and the general public.<br/>
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In the first system, our <b><a href="https://2013.igem.org/Team:KU_Leuven/HoneydewSystem"> honeydew system</a></b>, we spray our BanAphids directly onto the plants. In this system our BanAphids will <b>only produce MeS when aphids are present</b> because they are sensitive to the high concentrations of glucose found in the honeydew produced by the aphids. This interaction minimises the burden on the plant’s metabolism, attracts predators only when needed and avoids aphid habituation.<br/>
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<div class="span4 greytext video">
<div class="span4 greytext video">
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   <img src="https://static.igem.org/mediawiki/2013/0/07/Tekeningproject.jpg" alt="banaphids">
   <img src="https://static.igem.org/mediawiki/2013/0/07/Tekeningproject.jpg" alt="banaphids">
     <p><b>Honeydew model</b><br/> This drawing shows our BanAphids producing EBF to get rid of aphids and MeS to attract ladybugs.</p>
     <p><b>Honeydew model</b><br/> This drawing shows our BanAphids producing EBF to get rid of aphids and MeS to attract ladybugs.</p>
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       <h3>Honeydew model</h3>
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       <h3>Honeydew system</h3>
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   <p><iframe width="740" height="555" src="//www.youtube-nocookie.com/embed/Z2jOs70fEOs?rel=0" frameborder="0" allowfullscreen></iframe></p>
   <p><iframe width="740" height="555" src="//www.youtube-nocookie.com/embed/Z2jOs70fEOs?rel=0" frameborder="0" allowfullscreen></iframe></p>
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Secondly, we obtained early input from the private end user and we used this to design the <b>sticker system</b>.  
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We devised a second system with input from the private end user and we designed the <b>sticker system</b>.  
Our second method starts from the principle that the <b>BanAphids</b> may not come in direct contact with the plantsbecause of seafety concerns. For this we turned We turned to the <a href="https://2012.igem.org/Team:Groningen/Sticker">sticker model of of the Groningen 2012 iGEM team</a> to use as a container. The sticker is composed of a plastic film, whose pores are too small for the <b>BanAphids</b> to pass, but large enough so the volatile pheromones it produces still pass. The Groningen 2012 iGEM team has already investigated the biosafety of this sticker. However this means our system loses contact with the honeydew. To allow this containment and still avoid habituation we developed an oscillating system so that there is an  alternating MeS and EBF production.<br/><br/>
Our second method starts from the principle that the <b>BanAphids</b> may not come in direct contact with the plantsbecause of seafety concerns. For this we turned We turned to the <a href="https://2012.igem.org/Team:Groningen/Sticker">sticker model of of the Groningen 2012 iGEM team</a> to use as a container. The sticker is composed of a plastic film, whose pores are too small for the <b>BanAphids</b> to pass, but large enough so the volatile pheromones it produces still pass. The Groningen 2012 iGEM team has already investigated the biosafety of this sticker. However this means our system loses contact with the honeydew. To allow this containment and still avoid habituation we developed an oscillating system so that there is an  alternating MeS and EBF production.<br/><br/>

Revision as of 11:06, 28 October 2013

iGem

Secret garden

Congratulations! You've found our secret garden! Follow the instructions below and win a great prize at the World jamboree!


  • A video shows that two of our team members are having great fun at our favourite company. Do you know the name of the second member that appears in the video?
  • For one of our models we had to do very extensive computations. To prevent our own computers from overheating and to keep the temperature in our iGEM room at a normal level, we used a supercomputer. Which centre maintains this supercomputer? (Dutch abbreviation)
  • We organised a symposium with a debate, some seminars and 2 iGEM project presentations. An iGEM team came all the way from the Netherlands to present their project. What is the name of their city?

Now put all of these in this URL:https://2013.igem.org/Team:KU_Leuven/(firstname)(abbreviation)(city), (loose the brackets and put everything in lowercase) and follow the very last instruction to get your special jamboree prize!

tree ladybugcartoon

Our goal is to create a sustainable system to reduce the damage that aphids cause to our agricultural industry and reduce the use of insecticides.

The current solution usually requires huge amounts of insecticides, damaging ecosystems in several ways. First, the indiscriminate weakening of insects means that beneficial insects (pollinators, ...) are also affected. Second, insecticides affect vertebrates, including humans, damaging our health. Third, insecticide residues (catabolites) accumulate in each food-chain. The highest organism, be it humans or for example predatory birds, will end up with potentially high levels of residues and their negative effects.
We aim to offer an effective and sustainable alternative for insecticides. Our modified E. coli, the BanAphids (meaning ‘to ban aphids’ as well as provide plants with ‘benefits’), would produce the aphid’s own alarm pheromone, E-β-farnesene (EBF), to repel them off the plant. On top of that we want to attract aphid predators such as the ladybug via the production of Methyl Salicylate (MeS), a phytohormone, by our BanAphids. This way we ensure aphids are thoroughly removed from the plant.

We devised two different systems that might use these molecules. We did this because of response we received from both private end users and the general public.
In the first system, our honeydew system, we spray our BanAphids directly onto the plants. In this system our BanAphids will only produce MeS when aphids are present because they are sensitive to the high concentrations of glucose found in the honeydew produced by the aphids. This interaction minimises the burden on the plant’s metabolism, attracts predators only when needed and avoids aphid habituation.





banaphids

Honeydew model
This drawing shows our BanAphids producing EBF to get rid of aphids and MeS to attract ladybugs.

Honeydew system


We devised two different systems that might use these molecules. We did this because of response from both private end users and the general public.

In the first model, our honeydew model, we spray our BanAphids directly onto the plants. In this system our BanAphids will only produce MeS when aphids are present because they are sensitive to the high concentrations of glucose found in the honeydew produced by the aphids. This interaction minimises the burden on the plant’s metabolism, attracts predators only when needed and avoids habituation.

We devised a second system with input from the private end user and we designed the sticker system. Our second method starts from the principle that the BanAphids may not come in direct contact with the plantsbecause of seafety concerns. For this we turned We turned to the sticker model of of the Groningen 2012 iGEM team to use as a container. The sticker is composed of a plastic film, whose pores are too small for the BanAphids to pass, but large enough so the volatile pheromones it produces still pass. The Groningen 2012 iGEM team has already investigated the biosafety of this sticker. However this means our system loses contact with the honeydew. To allow this containment and still avoid habituation we developed an oscillating system so that there is an alternating MeS and EBF production.

Finally we want to define the impact of both these models on the ecosystem. In collaboration with relevant industries we performed various ecosystem experiments for the E.coligy part of our project. This allows us the demonstrate the effect of our models on both plants and insects.

"Sticker system"
This drawing shows how the sticker could be placed in a rose bush to repel aphids and attract ladybugs.

Aphid Background

Crashcourse in aphid biology

Honeydew System

Our BanAphids react to honeydew

Sticker System

An alternative approach

E. coligy

An autonomous alternative

Parts

BioBrick 'm all!

Data Page

All our achievements on one small page!