Team:Leeds/Parts

From 2013.igem.org

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Revision as of 02:40, 5 October 2013

Parts Summary

Silica Binding Peptide (Si4): A short amino acid sequence that binds silica with high affinity.
Bio-system 1: CpxR promoter controlling GFP expression. Will produce the green fluorescent protein following exposure to hydrophobic membrane stress. Extensive experimental data has been acquired about the response of the cpxR promoter to various forms of physical and chemical stresses, this can be found in the Device 1 section of the Lab Results page.
Bio-system 2: INP.Si4 is produced constitutively with GFP production under control of CpxR promoter. The system is designed to bind with high affinity to silica macro-structures and produce a fluorescent response when it does. Flow cytometry, fluorescence confocal, and scanning electron microscopy data has been gathered to characterize this part. Problems with expression meant that the data is not as conclusive as predicted but indicates that Si4 is expressed in some cells.

Submitted

A full listing of all parts Leeds iGEM 2013 have submitted to the Registry, and their status.
<groupparts>iGEM013 Leeds</groupparts>

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+==Parts Summary==
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-__NOTOC__
-{{Team:Leeds_layout|HeaderImage=[[File:Leeds_PartsHeader.png|825px|frameless]]|Header=Leeds' Registry Parts|content=
-Please check back here frequently for a full list of the parts we've developed, submitted to the [http://partsregistry.org Registry] and have had approved. We will also endeavour to include our characeterisation protocols, although we are currently collaborating with [[Team:Purdue|Purdue]] to decide exactly what these should be.
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-__TOC__
-==Currently Working On==
 * [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1028000 Silica Binding Peptide (Si4):] A short amino acid sequence that binds silica with high affinity.
-* [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1028002 Bio-system 1:] CpxR promoter controlling GFP expression. Will produce the green fluorescent protein following exposure to hydrophobic membrane stress. Extensive experimental data has been acquired about the response of the cpxR promoter to various forms of physical and chemical stresses, this can be found in the biosystem 1 section of the Lab Results page.
+* [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1028002 Bio-system 1:] CpxR promoter controlling GFP expression. Will produce the green fluorescent protein following exposure to hydrophobic membrane stress. Extensive experimental data has been acquired about the response of the cpxR promoter to various forms of physical and chemical stresses, this can be found in the Device 1 section of the Lab Results page.
-* [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1028003 Bio-system 2:] INP.Si4 is produced constitutively with GFP production under control of CpxR promoter. The system is designed to bind with high affinity to silica macro-structures and produce a fluorescent response when it does. Experimental data has been gathered for this part in unison with the silica binding peptide. Problems with expression meant that the data is not as conclusive as predicted but indicates that Si4 does bind silica with a degree of affinity.
+* [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1028003 Bio-system 2:] INP.Si4 is produced constitutively with GFP production under control of CpxR promoter. The system is designed to bind with high affinity to silica macro-structures and produce a fluorescent response when it does. Flow cytometry, fluorescence confocal, and scanning electron microscopy data has been gathered to characterize this part. Problems with expression meant that the data is not as conclusive as predicted but indicates that Si4 is expressed in some cells.
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 ==Submitted==