Team:NJU NJUT China

From 2013.igem.org

(Difference between revisions)
Line 849: Line 849:
$pxs_loading = $('.pxs_loading',$pxs_container),
$pxs_loading = $('.pxs_loading',$pxs_container),
$pxs_slider_wrapper = $('.pxs_slider_wrapper',$pxs_container);
$pxs_slider_wrapper = $('.pxs_slider_wrapper',$pxs_container);
-
 
-
//first preload all the images
 
var loaded = 0,
var loaded = 0,
$images = $pxs_slider_wrapper.find('img');
$images = $pxs_slider_wrapper.find('img');
Line 864: Line 862:
//one images width (assuming all images have the same sizes)
//one images width (assuming all images have the same sizes)
var one_image_w = $pxs_slider.find('img:first').width();
var one_image_w = $pxs_slider.find('img:first').width();
-
 
-
/*
 
-
need to set width of the slider,
 
-
of each one of its elements, and of the
 
-
navigation buttons
 
-
*/
 
setWidths($pxs_slider,
setWidths($pxs_slider,
$elems,
$elems,
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});
});
}
}
-
//hovering the thumbs animates them up and down
 
$this.bind('mouseenter',function(){
$this.bind('mouseenter',function(){
$(this).stop().animate({top:'-10px'},100);
$(this).stop().animate({top:'-10px'},100);
Line 905: Line 896:
});
});
-
//make the first thumb be selected
 
highlight($thumbs.eq(0));
highlight($thumbs.eq(0));
-
 
-
//slide when clicking the navigation buttons
 
$pxs_next.bind('click',function(){
$pxs_next.bind('click',function(){
++current;
++current;
Line 947: Line 935:
o.easingBg);
o.easingBg);
});
});
-
 
-
/*
 
-
clicking a thumb will slide to the respective image
 
-
*/
 
$thumbs.bind('click',function(){
$thumbs.bind('click',function(){
var $thumb = $(this);
var $thumb = $(this);
Line 967: Line 951:
o.easingBg);
o.easingBg);
});
});
-
 
-
 
-
 
-
/*
 
-
activate the autoplay mode if
 
-
that option was specified
 
-
*/
 
if(o.auto != 0){
if(o.auto != 0){
o.circular = true;
o.circular = true;
Line 980: Line 957:
},o.auto);
},o.auto);
}
}
-
 
-
/*
 
-
when resizing the window,
 
-
we need to recalculate the widths of the
 
-
slider elements, based on the new windows width.
 
-
we need to slide again to the current one,
 
-
since the left of the slider is no longer correct
 
-
*/
 
$(window).resize(function(){
$(window).resize(function(){
w_w = $(window).width();
w_w = $(window).width();
Line 1,052: Line 1,021:
$pxs_next,
$pxs_next,
$pxs_prev){
$pxs_prev){
-
/*
 
-
the width of the slider is the windows width
 
-
times the total number of elements in the slider
 
-
*/
 
var pxs_slider_w = w_w * total_elems;
var pxs_slider_w = w_w * total_elems;
$pxs_slider.width(pxs_slider_w + 'px');
$pxs_slider.width(pxs_slider_w + 'px');
//each element will have a width = windows width
//each element will have a width = windows width
$elems.width(w_w + 'px');
$elems.width(w_w + 'px');
-
/*
 
-
we also set the width of each bg image div.
 
-
The value is the same calculated for the pxs_slider
 
-
*/
 
$pxs_bg1.width(pxs_slider_w + 'px');
$pxs_bg1.width(pxs_slider_w + 'px');
$pxs_bg2.width(pxs_slider_w + 'px');
$pxs_bg2.width(pxs_slider_w + 'px');
$pxs_bg3.width(pxs_slider_w + 'px');
$pxs_bg3.width(pxs_slider_w + 'px');
-
 
-
/*
 
-
both the right and left of the
 
-
navigation next and previous buttons will be:
 
-
windowWidth/2 - imgWidth/2 + some margin (not to touch the image borders)
 
-
*/
 
var position_nav = w_w/2 - one_image_w/2 + 3;
var position_nav = w_w/2 - one_image_w/2 + 3;
$pxs_next.css('right', position_nav + 'px');
$pxs_next.css('right', position_nav + 'px');
Line 1,135: Line 1,090:
<script>
<script>
jQuery.easing['jswing'] = jQuery.easing['swing'];
jQuery.easing['jswing'] = jQuery.easing['swing'];
-
 
jQuery.extend( jQuery.easing,
jQuery.extend( jQuery.easing,
{
{

Revision as of 16:13, 25 August 2013

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Project

Basic Information

    Most of the bacteria and archaea acquire virus resistance by integrating short viral nucleotide acid fragments into the clusters of regularly interspaced short palindromic repeats (CRISPRs). And CRISPR-based defense system can also protects them against the invading DNA and/or RNA elements. It is believed that the integrated CRISPR sequences have the ability to form a genetic memory which prevents the host from being infected. The CRISPRs and Cas (CRISPR-associated) interact and form this prokaryotic adaptive immune system....

 

Introduction to our experiment

information

Based on the fundamental synthetic biology methods, include the molecular cloning, long PCR, recombinant construction, restriction digestion and yeast transformation, our project aims to enable Saccharomyces cerevisiae to resist viruses utilizing the CRISPR system found in most bacteria and archaea lately.

To ensure the accurate output, we divide the original linear gene into several elements , and also purify and propagate the complete target sequence to make the both two models work in the host cell. To present the actual effect of CRISPR in yeast, we design our own mimic virus construction based on the homologous sequences shown in the specific viral genome database, which can be recognized, and control the baseline both for anti-virus ability and CRISPR's effect in its original form.

More specifically, we first obtain our CRISPR system by genome-extraction from E.coli str W3110, and clone the respective target genes as modules. The construction, as well as the mimic virus construction, IS made by restriction-ligase way. We transform the mimic virus plasmid first by lithium acetate method and verify the availability by several antibiotics. We then import CRISPR system in the same way, utilizing the Ochre mutation and GFP switch-off to evaluate the anti-virus feasibility.

Read More

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