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| - | <div id="top-section3"><center><img src="https://static.igem.org/mediawiki/2013/5/55/Logo-ntnu.png" alt="header" border="0" usemap="#igemmap" width="1000" height="400"></center></div>
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| - | <p>Biosensors have widespread applications ranging from diagnostics to environmental monitoring. Vibrio cholerae's ToxR system can be used as a component in biological sensing devices. ToxS causes ToxR homodimerization, activating transcription of the ctx promoter. By replacing the periplasmic domain of ToxR with existing or engineered ligand-dependent homodimers, we hope to link ToxR dimerization (and gene expression) to the presence of specific ligands. Initially, ToxR constructs proved toxic to E. coli. We built a stress-regulated transcription system that drives relatively high expression of toxic proteins. This allowed us to further engineer ToxR chimeras. We fused an estrogen-dependent dimer with ToxR hoping to create an estrogen biosensor. We observed a range of constitutive phenotypes and plan more experiments to engineer a dose-dependent transcriptional response to estrogen. By fusing existing or engineered ligand dependent homodimers to ToxR, this modular system can be used to build new biosensors. </p>
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| - | <div class="col4"style="background-color:#DD87C0;"><a href="https://2011.igem.org/Team:Berkeley/Project#ToxR"> <img src="https://static.igem.org/mediawiki/igem.org/1/10/Subtitlepic1.jpg"
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| - | <p style="text-align:center; color:#1A1719; "> Vesicle project </p> </div>
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| - | <div class="col4"style="background-color:#185f73;"><a href="https://2011.igem.org/Team:Berkeley/Project#ToxRChimera"><img src="https://static.igem.org/mediawiki/2011/b/bb/Subtitlepic3header.jpg"
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| - | <p style="text-align:center; color:#CECECE;"> Fluorescence protein dimers </p></div>
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| - | <div class="col4" style="background-color:#303285"><a href="https://2011.igem.org/Team:Berkeley/Project#StressPromoters"><img src="https://static.igem.org/mediawiki/2011/2/2b/Subtitlepic2header.jpg"
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| - | <p style="text-align:center; color:#CECECE;"> PmXylS promoter </p> </div>
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