http://2013.igem.org/wiki/index.php?title=Team:NTNU-Trondheim&feed=atom&action=historyTeam:NTNU-Trondheim - Revision history2024-03-29T09:14:45ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:NTNU-Trondheim&diff=306722&oldid=prevMariakan at 22:50, 4 October 20132013-10-04T22:50:32Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center; color:black; "> <b> About us</b></p> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center; color:black; "> <b> About us</b></p> </div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>This is the third year a team from the Norwegian University of Science and Technology (NTNU) is participating in the iGEM competition. Our team consists of five students with background in Biotechnology, Molecular Medicine and Medical Technology. Mostly our collective background is in molecular biology colored with biophysics, immunology and biotechnology. We have four advisors with different specialities! Rahmi is our lab-GOD and has all the answers. Eivind gives us money and inspiration, Martin is our GFP-expert and Gunvor with her past iGEM team experience has been a lot of help! We are naive students that want to come up with crazy ideas that supervisors are to experienced to consider an opportunity. Our project this year is HIGH-risk and it was almost immediately clear to us that this was what we wanted to do this year. Considering the time-limit of iGEM it was an ambitious project and had everything gone as planned and expected we could have reached our goal. We <del class="diffchange diffchange-inline">still </del>believe the project has potential and should <del class="diffchange diffchange-inline">not </del>be <del class="diffchange diffchange-inline">abandoned</del>. We'<del class="diffchange diffchange-inline">we </del>all enjoyed working as a team and learned tons about synthetic biology and the potential this field has feels unlimited! We have had a lot of fun, the life of a scientist is like a rollercoaster of <del class="diffchange diffchange-inline">up's </del>and <del class="diffchange diffchange-inline">down's </del>but the excitement and curiosity of research will always be a strong motivator!</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>This is the third year a team from the Norwegian University of Science and Technology (NTNU) is participating in the iGEM competition. Our team consists of five students with background in Biotechnology, Molecular Medicine and Medical Technology. Mostly our collective background is in molecular biology colored with biophysics, immunology and biotechnology. We have four advisors with different specialities! Rahmi is our lab-GOD and has all the answers. Eivind gives us money and inspiration, Martin is our GFP-expert and Gunvor with her past iGEM team experience has been a lot of help! We are naive students that want to come up with crazy ideas that supervisors are to experienced to consider an opportunity. Our project this year is HIGH-risk and it was almost immediately clear to us that this was what we wanted to do this year. Considering the time-limit of iGEM it was an ambitious project and had everything gone as planned and expected we could have reached our goal. We believe the project has potential and <ins class="diffchange diffchange-inline">it </ins>should be <ins class="diffchange diffchange-inline">further investigated</ins>. We'<ins class="diffchange diffchange-inline">ve </ins>all enjoyed working as a team and learned tons about synthetic biology and the potential this field has feels unlimited! We have had a lot of fun, the life of a scientist is like a rollercoaster of <ins class="diffchange diffchange-inline">ups </ins>and <ins class="diffchange diffchange-inline">downs </ins>but the excitement and curiosity of research will always be a strong motivator!</p></div></td></tr>
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</table>Mariakanhttp://2013.igem.org/wiki/index.php?title=Team:NTNU-Trondheim&diff=305736&oldid=prevIngridfa at 22:21, 4 October 20132013-10-04T22:21:48Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We are going to prove that the OMVs can be manipulated by introducing two different proteins. The first one is a fusion protein of GFP and RFP. This will make it possible to visualize the vesicles as well as investigating the new functionalities of fusing two fluorescent proteins together. The second protein we wish to introduce to the vesicles is the transmembrane protein G derived from <i> Streptococcus dysgalactiae ssp. equisimilisi</i>. Protein G is known to bind to Human Serum Albumin (HSA) which helps <i>S.dysgalactiae subsp. equisimilis</i> hide from the immune system. Protein G will therefore be a potential important piece in a drug carrier by masking it from immunological destruction and making it stable in the blood stream. <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We are going to prove that the OMVs can be manipulated by introducing two different proteins. The first one is a fusion protein of GFP and RFP. This will make it possible to visualize the vesicles as well as investigating the new functionalities of fusing two fluorescent proteins together. The second protein we wish to introduce to the vesicles is the transmembrane protein G derived from <i> Streptococcus dysgalactiae ssp. equisimilisi</i>. Protein G is known to bind to Human Serum Albumin (HSA) which helps <i>S.dysgalactiae subsp. equisimilis</i> hide from the immune system. Protein G will therefore be a potential important piece in a drug carrier by masking it from immunological destruction and making it stable in the blood stream. <br><br></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In order to direct the proteins into the periplasm and vesicles they need to have a tat signal peptide at the N-terminal. This will transport the proteins through twin-arginine translocation pathway (Tat pathway. Studies show that a twin-arginine signal peptide is able to direct the export of active green fluorescent protein (GFP) in <i> Escherichia coli </i>. We also wish to be able to regulate the production and thereby the export of <del class="diffchange diffchange-inline">protein </del>to OMVs. In order to accomplish this task we will set the gene constructs under regulation of the Pm/Xyls promotor system. This is a positive regulation system that <del class="diffchange diffchange-inline">gets </del>activated by the inducer m-toluic acid<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In order to direct the proteins into the periplasm and vesicles they need to have a tat signal peptide at the N-terminal. This will transport the proteins through twin-arginine translocation pathway (Tat pathway<ins class="diffchange diffchange-inline">)</ins>. Studies show that a twin-arginine signal peptide is able to direct the export of active green fluorescent protein (GFP) in <i> Escherichia coli </i>. We also wish to be able to regulate the production and thereby the export of <ins class="diffchange diffchange-inline">proteins </ins>to OMVs. In order to accomplish this task we will set the gene constructs under regulation of the Pm/Xyls promotor system. This is a positive regulation system that <ins class="diffchange diffchange-inline">is </ins>activated by the inducer m-toluic acid<br><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Introducing these proteins into vesicles also demonstrate that it is indeed possible to manipulate the content and therefore the properties of OMV's. We will prove the concept that OMVs can be engineered to meet the criteria of a drug delivery vehicle. <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Introducing these proteins into vesicles also demonstrate that it is indeed possible to manipulate the content and therefore the properties of OMV's. We will prove the concept that OMVs can be engineered to meet the criteria of a drug delivery vehicle. <br><br></div></td></tr>
</table>Ingridfahttp://2013.igem.org/wiki/index.php?title=Team:NTNU-Trondheim&diff=305189&oldid=prevMariakan at 22:03, 4 October 20132013-10-04T22:03:01Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center; color:black; "><b> The project</b> </p> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center; color:black; "><b> The project</b> </p> </div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><del class="diffchange diffchange-inline">Gram </del>negative bacteria <del class="diffchange diffchange-inline">export numerous proteins into the periplasm. The twin-arginine translocation pathway </del>(<del class="diffchange diffchange-inline">Tat pathway</del>) <del class="diffchange diffchange-inline">is a protein export</del>, <del class="diffchange diffchange-inline">or secretion pathway found </del>in <del class="diffchange diffchange-inline">plants, bacteria, </del>and <del class="diffchange diffchange-inline">archaea. </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><ins class="diffchange diffchange-inline">All gram </ins>negative bacteria <ins class="diffchange diffchange-inline">produce outer membrane vesicles </ins>(<ins class="diffchange diffchange-inline">OMVs</ins>) <ins class="diffchange diffchange-inline">by bulging of from their outer membrane. These OMVs contain different proteins and carry out varies functions such as quorum sensing</ins>, <ins class="diffchange diffchange-inline">involvement </ins>in <ins class="diffchange diffchange-inline">pathogenesis </ins>and <ins class="diffchange diffchange-inline">transporting enzymes </ins>to <ins class="diffchange diffchange-inline">distal locations</ins>. <ins class="diffchange diffchange-inline">In our project </ins>we <ins class="diffchange diffchange-inline">wish </ins>to <ins class="diffchange diffchange-inline">prove that OMVs can be manipulated and to thereby implicate OMVs potential as a drug delivery vehicle. This is an innovative and <a href="https://2013</ins>.<ins class="diffchange diffchange-inline">igem.org/Team:NTNU-Trondheim/novelapproach"> novel approach </a></ins>to <ins class="diffchange diffchange-inline">create a safe way to deliver drugs </ins>in the <ins class="diffchange diffchange-inline">body</ins>. <br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> The Tat pathway serves </del>to <del class="diffchange diffchange-inline">actively translocate folded proteins across a lipid membrane bilayer</del>. <del class="diffchange diffchange-inline">By using this transportationsystem </del>we <del class="diffchange diffchange-inline">aim </del>to <del class="diffchange diffchange-inline">introduce new proteins into the periplasm of bacteria</del>. <del class="diffchange diffchange-inline">Once in the periplasm the protein will </del>to <del class="diffchange diffchange-inline">some extent end up </del>in <del class="diffchange diffchange-inline">outer membrane vesicles (OMV's) that budd of </del>the <del class="diffchange diffchange-inline">bacteria</del>.<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">As all gram negative bacteria produce outer membrane vesicles, we looked into </del>the <del class="diffchange diffchange-inline">content </del>of <del class="diffchange diffchange-inline">these vesicles</del>. <del class="diffchange diffchange-inline">Was </del>the <del class="diffchange diffchange-inline">sorting </del>of proteins <del class="diffchange diffchange-inline">random? Could </del>we <del class="diffchange diffchange-inline">direct certain proteins toward them? And what function would that give? Can we use this </del>to <del class="diffchange diffchange-inline">our advantage?</del><br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">We are going to prove that </ins>the <ins class="diffchange diffchange-inline">OMVs can be manipulated by introducing two different proteins. The first one is a fusion protein </ins>of <ins class="diffchange diffchange-inline">GFP and RFP</ins>. <ins class="diffchange diffchange-inline">This will make it possible to visualize </ins>the <ins class="diffchange diffchange-inline">vesicles as well as investigating the new functionalities </ins>of <ins class="diffchange diffchange-inline">fusing two fluorescent </ins>proteins <ins class="diffchange diffchange-inline">together. The second protein </ins>we <ins class="diffchange diffchange-inline">wish </ins>to <ins class="diffchange diffchange-inline">introduce to the vesicles is the transmembrane protein G derived from <i> Streptococcus dysgalactiae ssp. equisimilisi</i>. Protein G is known to bind to Human Serum Albumin (HSA) which helps <i>S.dysgalactiae subsp. equisimilis</i> hide from the immune system. Protein G will therefore be a potential important piece in a drug carrier by masking it from immunological destruction and making it stable in the blood stream. </ins><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Studies show that a twin-arginine signal peptide is able to direct the export of active green fluorescent protein (GFP) in <del class="diffchange diffchange-inline">E.</del>coli and <del class="diffchange diffchange-inline">that translocation almost exclusively occur by </del>the <del class="diffchange diffchange-inline">Tat-pathway</del>. <del class="diffchange diffchange-inline">With </del>this <del class="diffchange diffchange-inline">in mind </del>we <del class="diffchange diffchange-inline">proceeded with making a construct containing tat and a GFP.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">In order to direct the proteins into the periplasm and vesicles they need to have a tat signal peptide at the N-terminal. This will transport the proteins through twin-arginine translocation pathway (Tat pathway. </ins>Studies show that a twin-arginine signal peptide is able to direct the export of active green fluorescent protein (GFP) in <ins class="diffchange diffchange-inline"><i> Escherichia </ins>coli <ins class="diffchange diffchange-inline"></i>. We also wish to be able to regulate the production </ins>and <ins class="diffchange diffchange-inline">thereby </ins>the <ins class="diffchange diffchange-inline">export of protein to OMVs</ins>. <ins class="diffchange diffchange-inline">In order to accomplish </ins>this <ins class="diffchange diffchange-inline">task </ins>we <ins class="diffchange diffchange-inline">will set </ins>the <ins class="diffchange diffchange-inline">gene constructs under regulation </ins>of the <ins class="diffchange diffchange-inline">Pm/Xyls promotor system. This </ins>is <ins class="diffchange diffchange-inline">a positive regulation system that gets activated by </ins>the <ins class="diffchange diffchange-inline">inducer m-toluic acid</ins><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">As </del>the <del class="diffchange diffchange-inline">goal </del>of the <del class="diffchange diffchange-inline">project </del>is <del class="diffchange diffchange-inline">to determine if vesicles can be utilized for drug delivery we want to see if they can be masked from </del>the <del class="diffchange diffchange-inline">immunesystem by introducing a specific protein.</del><br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Protein G is known to bind to Human Serum Albumin which helps S.dysgalactiae subsp. equisimilis hide from the immune system. Protein G could therefore be a potential important piece in a drug carrier by masking it from immunological destruction. </del>Introducing <del class="diffchange diffchange-inline">protein G </del>into vesicles also demonstrate that it is indeed possible to manipulate the content and therefore the properties of OMV's. <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Introducing <ins class="diffchange diffchange-inline">these proteins </ins>into vesicles also demonstrate that it is indeed possible to manipulate the content and therefore the properties of OMV's. <ins class="diffchange diffchange-inline">We will prove the concept that OMVs can be engineered to meet the criteria of a drug delivery vehicle. <br></ins><br></div></td></tr>
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</table>Mariakanhttp://2013.igem.org/wiki/index.php?title=Team:NTNU-Trondheim&diff=304682&oldid=prevEllens at 21:44, 4 October 20132013-10-04T21:44:12Z<p></p>
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</table>Ellenshttp://2013.igem.org/wiki/index.php?title=Team:NTNU-Trondheim&diff=304662&oldid=prevEllens at 21:43, 4 October 20132013-10-04T21:43:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center; color:black; "><b> The project</b> </p> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center; color:black; "><b> The project</b> </p> </div></div></td></tr>
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</table>Ellenshttp://2013.igem.org/wiki/index.php?title=Team:NTNU-Trondheim&diff=304512&oldid=prevShivash at 21:38, 4 October 20132013-10-04T21:38:32Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li <del class="diffchange diffchange-inline">class='active'</del>><a href='https://2013.igem.org/Team:NTNU-Trondheim'><span>Home</span></a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href='https://2013.igem.org/Team:NTNU-Trondheim'><span>Home</span></a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class='has-sub'><a href='#'><span>Project</span></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class='has-sub'><a href='#'><span>Project</span></a></div></td></tr>
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</table>Shivashhttp://2013.igem.org/wiki/index.php?title=Team:NTNU-Trondheim&diff=304430&oldid=prevIngridfa at 21:35, 4 October 20132013-10-04T21:35:13Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><a href="https://2013.igem.org/Team:NTNU-Trondheim/Achievements">Medal criteria</a><br></ins></div></td></tr>
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</table>Ingridfahttp://2013.igem.org/wiki/index.php?title=Team:NTNU-Trondheim&diff=303923&oldid=prevEllens at 21:14, 4 October 20132013-10-04T21:14:14Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p style="text-align:center; color:black; "> <b>Vesicle project</b> </p> </div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p style="text-align:center; color:black; "> <b<ins class="diffchange diffchange-inline">><a href="https://2013.igem.org/Team:NTNU-Trondheim/Project"</ins>>Vesicle project<ins class="diffchange diffchange-inline"></a></ins></b> </p> </div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="text-align:center; color:black;"><b> Pm/XylS promoter</b> </p> </div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="text-align:center; color:black;"><b<ins class="diffchange diffchange-inline">><a href="https://2013.igem.org/Team:NTNU-Trondheim/Project"</ins>> Pm/XylS promoter<ins class="diffchange diffchange-inline"></a></ins></b> </p> </div></div></td></tr>
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</table>Ellenshttp://2013.igem.org/wiki/index.php?title=Team:NTNU-Trondheim&diff=303750&oldid=prevEllens at 21:07, 4 October 20132013-10-04T21:07:36Z<p></p>
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</table>Ellenshttp://2013.igem.org/wiki/index.php?title=Team:NTNU-Trondheim&diff=303662&oldid=prevIngridfa at 21:03, 4 October 20132013-10-04T21:03:52Z<p></p>
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