Team:NTNU-Trondheim/Notebook

From 2013.igem.org

(Difference between revisions)
(Tuesday 18.06.13)
(Tuesday 18.06.13)
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Started transforming BioBricks. Transformed some composite BioBricks consisting of constitutive promotor, RBS, a fluorescent protein and a terminator. Transformed the parts <partinfo>BBa_I13521</partinfo>, <partinfo>BBa_I13522</partinfo>, and <partinfo>BBa_I13600</partinfo> to competent ''E.coli'' DH5&alpha; cells, to have some colorful cells to show the camera crew from NRK (Norwegian broadcasting), who will visit our lab tomorrow :-)
Started transforming BioBricks. Transformed some composite BioBricks consisting of constitutive promotor, RBS, a fluorescent protein and a terminator. Transformed the parts <partinfo>BBa_I13521</partinfo>, <partinfo>BBa_I13522</partinfo>, and <partinfo>BBa_I13600</partinfo> to competent ''E.coli'' DH5&alpha; cells, to have some colorful cells to show the camera crew from NRK (Norwegian broadcasting), who will visit our lab tomorrow :-)
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For our transformation protocol, se the [https://2013.igem.org/Team:NTNU-Trondheim/Protocols protocol page].  
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For our transformation protocol, se the [https://2013.igem.org/Team:NTNU-Trondheim/Protocols protocol page].
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====Wednesday 19.06.13====
====Wednesday 19.06.13====

Revision as of 08:05, 21 June 2013

NTNU Trondheim


January
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February
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April
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May
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June
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August
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September
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October
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November
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December
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Notebook

We transferred the transformed cells from yesterday to liquid medium and incubated at 37°C protocol.

We isolated the DNA from the transformed mCherry cells by using the miniprep protocol and measured the concentration by NanoDrop ND-1000 Spectrophotometer.

Sample Concentration (ng/µl)
mCherry1 5,6
mCherry2 7,3


Tuesday 18.06.13

Started transforming BioBricks. Transformed some composite BioBricks consisting of constitutive promotor, RBS, a fluorescent protein and a terminator. Transformed the parts <partinfo>BBa_I13521</partinfo>, <partinfo>BBa_I13522</partinfo>, and <partinfo>BBa_I13600</partinfo> to competent E.coli DH5α cells, to have some colorful cells to show the camera crew from NRK (Norwegian broadcasting), who will visit our lab tomorrow :-)

For our transformation protocol, se the protocol page.


Wednesday 19.06.13


Retrieved from "http://2013.igem.org/Team:NTNU-Trondheim/Notebook"