We transferred the transformed cells from yesterday to liquid medium and incubated at 37°C protocol.
We isolated the DNA from the transformed mCherry cells by using the miniprep protocol and measured the concentration by NanoDrop ND-1000 Spectrophotometer.
Sample | Concentration (ng/µl) |
mCherry1 | 5,6 |
mCherry2 | 7,3 |
Started transforming BioBricks. Transformed some composite BioBricks consisting of constitutive promotor, RBS, a fluorescent protein and a terminator. Transformed the parts <partinfo>BBa_I13521</partinfo>, <partinfo>BBa_I13522</partinfo>, and <partinfo>BBa_I13600</partinfo> to competent E.coli DH5α cells, to have some colorful cells to show the camera crew from NRK (Norwegian broadcasting), who will visit our lab tomorrow :-)
For our transformation protocol, se the protocol page.