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Team:Paris Bettencourt/Notebook/TB-ception/Friday 13th September.html
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A lot of macrophages died (in suspension) because we forgot to add glutamine and pyruvate sodium. We repeated the experiment with the complete media (but again without antibiotics). | A lot of macrophages died (in suspension) because we forgot to add glutamine and pyruvate sodium. We repeated the experiment with the complete media (but again without antibiotics). | ||
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| + | <br />• Results for the GA: because the Chl plates were off, I have to repeat everything for biobricking TDMH and for the pACYC derived backbone. The cloning for pCDF and pColA worked - patched the colonies, start liquid cultures. | ||
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| + | <br />• Launch NEB Turbo to make competent cells. | ||
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Latest revision as of 14:28, 25 September 2013
TB-ception
ASDFFriday 13th September
A lot of macrophages died (in suspension) because we forgot to add glutamine and pyruvate sodium. We repeated the experiment with the complete media (but again without antibiotics).
• Results for the GA: because the Chl plates were off, I have to repeat everything for biobricking TDMH and for the pACYC derived backbone. The cloning for pCDF and pColA worked - patched the colonies, start liquid cultures.
• Launch NEB Turbo to make competent cells.
