Team:Poznan-BioInf/Introduction
From 2013.igem.org
(tekst01) |
|||
Line 41: | Line 41: | ||
<div class="well"> | <div class="well"> | ||
- | + | <h4>Overview</h4> | |
<p> | <p> | ||
- | + | Our project aims to construct a synthetic biological device based on a digital circuit called a multiplexer. Our system is designed to generate | |
+ | a response dependent solely on the provided input signals. As multiple input signals were needed, we had to test multiple inducible promoters. Activities | ||
+ | of these promoters are induced by presence or absence of various monosaccharides: xylose, arabinose, rhamnose and disaccharide melibiose. | ||
+ | Thus, their concentrations were equivalent to analog signals that could be converted to expression of serine recombinases (phage integrases) | ||
+ | in an 'all-or-none' way, providing us with a digital control over the system. | ||
+ | </p><p> | ||
+ | Using the interases' ability to recognize nonidentical sites and invert or excise identified sequence, one may make DNA editable in vivo. | ||
+ | One of the possible uses of such a sub-system is to create a biological equivalent of a transistor - dubbed a transcriptor - that uses DNA polimerase flow as an analogue of the electric current, | ||
+ | while exploiting the integrase as a control signal. That suffices to build an SR-latch - the simplest memory cell, being one of the basic units of the Von Neumann computer architecture. | ||
+ | The resultant DNA-based memory storage can be propagated when cells divide. | ||
+ | </p> | ||
+ | <p> | ||
+ | We envision that the abilities of integrases could allow switching expression | ||
+ | on and off, targeting various genetic components. | ||
+ | |||
+ | |||
+ | |||
</p> | </p> |
Revision as of 23:40, 4 October 2013