Team:Queens Canada/Project/Repel

From 2013.igem.org

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        AtoE is a membrane transporter of short chain fatty acids. This gene is normally expressed in E. coli but in our project, the plan is to constitutively express this gene such that it is constantly uptaking surrounding isovaleric acid. Once within the bacteria, other reactions ensue.  
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AtoE is a non-specific membrane transporter of short-chain fatty acids endogenously expressed in E. coli. High constitutive expression of this gene would allow high intake of surrounding isovaleric acid and channelling into the breakdown pathway.
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In order to isolate the gene from the E. coli genome, we designed PCR primers such that the AtoE gene could be replicated with the biobrick cut sites on the ends of it. The following
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Revision as of 03:43, 27 September 2013

Untitled Document

QGEM 2013 PRESENTS:
Mosquito Repellent
Our idea for a mosquito repellent is to neutralize the odours that mosquitoes are attracted to. Recent studies have shown that malarial mosquitoes rely 4x more heavily on their sense of smell to find their prey. By removing the volatile compounds that cause these smells, we are effectively able to decrease the ability of mosquitoes to find humans. To achieve this end, we have created a genetic and enzymatic pathway. It begins with the uptake of isovaleric acid, the smell inducing compound, and converts it into a pleasant banana odour. Domestically, this serves as a foot deodorant but on a global scale it may also curb malaria rates.
AtoE
AtoE is a non-specific membrane transporter of short-chain fatty acids endogenously expressed in E. coli. High constitutive expression of this gene would allow high intake of surrounding isovaleric acid and channelling into the breakdown pathway.

In order to isolate the gene from the E. coli genome, we designed PCR primers such that the AtoE gene could be replicated with the biobrick cut sites on the ends of it. The following
NCAR and NPT
Carboxylic acid reductase (CAR) is an enzyme that converts fatty acids such as isovaleric into aldehydes. Phosphopantetheinyl transferase (NPT) is an enzyme that induces a post-translational modification by adding a pantetheinyl arm to the active site of the carboxylic acid reductase, which is crucial for the formation of a thioester intermediate.
YjgB
YjgB is a catalytic enzyme that converts aldehyde into alcohol.
ATF1
Acetyl transferase 1 (ATF1) was a BioBrick created by MIT in 2006 as part of their iGEM project. It is derived from Saccharomyces cerevisiae and it catalyzes the conversion of isoamyl alcohol to isoamyl acetate, a compound that gives off a banana scent. This is the final step of our pathway, completing the neutralization of isovaleric acid.
A huge thanks to our sponsors: