Team:SCU China
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Synthetic multicellular system has always been one of the most interesting parts of syn-bio.In the fundamental researches, especially the imitations of the whole developing procedure from a single cell to a self-organized multicellular system, many pervious iGEM teams have made plenty of explorations. | Synthetic multicellular system has always been one of the most interesting parts of syn-bio.In the fundamental researches, especially the imitations of the whole developing procedure from a single cell to a self-organized multicellular system, many pervious iGEM teams have made plenty of explorations. | ||
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However, there haven’t been comprehensive study into the imitations of sex differentiation, gametogenesis, and sexual reproduction. | However, there haven’t been comprehensive study into the imitations of sex differentiation, gametogenesis, and sexual reproduction. | ||
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- | + | <span style="color: #ff0000;font-weight:bold;float:left">In our project,we intend to construct two groups of differentiated E.coli,one imitates the male multicellular organism ,the other for the female(called G<sup>+</sup> cells and G<sup>-</sup> cells respectively).</span> | |
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*When cultured separately, the male/female multicellular system gets bigger and matures, and cells will differentiate into gametes,which cannot divide any more but are capable of gene transfer. | *When cultured separately, the male/female multicellular system gets bigger and matures, and cells will differentiate into gametes,which cannot divide any more but are capable of gene transfer. | ||
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- | *After that, you mix this two liquid cultures,the male gametes will recognize the female cells and begin to transfer modified F plasmids into female gametes through sex pili. The conjugation makes female gametes return to the state of un-differentiation(called G cells),which means they can divide again but are not sexually determined. | + | *After that, you mix this two liquid cultures,the male gametes will recognize the female cells and begin to transfer modified F plasmids into female gametes through sex pili. The conjugation makes female gametes return to the state of un-differentiation(called G cells),which means they can divide again but are not sexually determined. |
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*Then,after several cell divisions,one G cell will differentiate into a G<sup>+</sup> or G<sup>-</sup>,which, like zygote , can grow into next generation of the multicellular system maybe containing genes from both male and female gametes. | *Then,after several cell divisions,one G cell will differentiate into a G<sup>+</sup> or G<sup>-</sup>,which, like zygote , can grow into next generation of the multicellular system maybe containing genes from both male and female gametes. | ||
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Latest revision as of 15:52, 13 October 2013