Team:SCU China

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Project Description

Synthetic multicellular system has always been one of the most interesting parts of syn-bio.In the fundamental researches, especially the imitations of the whole developing procedure from a single cell to a self-organized multicellular system, many pervious iGEM teams have made plenty of explorations.

However, there haven’t been comprehensive study into the imitations of sex differentiation, gametogenesis, and sexual reproduction.

  • In our project,we intend to construct two groups of differentiated E.coli,one imitates the male multicellular organism ,the other for the female(called G+ cells and G- cells respectively).
  • When cultured separately, the male/female multicellular system gets bigger and matures, and cells will differentiate into gametes,which cannot divide any more but are capable of gene transfer.
  • After that, you mix this two liquid cultures,the male gametes will recognize the female cells and begin to transfer modified F plasmids into female gametes through sex pili. The conjugation makes female gametes return to the state of un-differentiation(called G cells),which means they can divide again but are not sexually determined.
  • Then,after several cell divisions,one G cell will differentiate into a G+ or G-,which, like zygote , can grow into next generation of the multicellular system maybe containing genes from both male and female gametes.
File:SCU-index1
SCU-cartoon.JPG