From 2013.igem.org
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| - | == '''Brief Project Description''' ==
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| - | '''Background'''
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| - | *1,2-dichloroethane (DCA) is part of a family of chlorinated hydrocarbons that are primarily derived from industrial solvents such as tetrachloroethene (PCE) and trichloroethene (TCE). Some of these organochlorines are toxic, carcinogenic and generally nasty.
| + | We’re a team of undergraduates from Sydney University. This is almost all of us - |
| - | *DCA is a soluble and mobile contaminant of the groundwater in Botany Bay, Sydney, but also elsewhere around the world.
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| - | *Conventional treatment involves significant costs, such as the pumping and heat-stripping of groundwater at Botany Bay. A biological alternative may be cheaper and more effective.
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| - | *A suite of techniques (including sampling of contaminated sites, growth in bioreactors with selective conditions and protein engineering) have led to an understanding that there are two primary pathways of DCA-degradation (Fig. 1).
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| - | '''Goals'''
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| - | *Construct a BioBrick-compatible vector inspired by the broad host range vector, pBBR1MCS2.
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| - | *Construct and compare two of the proposed pathways of DCA biodegradation.
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| - | *Characterise the components of the DCA-degradation pathway for admission into the Registry of Standard Parts.
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| - | *(If we get time!) Demonstrate the integration of our the pathway in the chromosome of Pseudomonas stutzeri via natural transformation and site-specific recombination.
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| | [[File:SydneyUni_2013_Home_Team.jpg|centre]] | | [[File:SydneyUni_2013_Home_Team.jpg|centre]] |
| | {{Team:SydneyUni_Australia/Footer}} | | {{Team:SydneyUni_Australia/Footer}} |
Revision as of 08:15, 27 September 2013
We’re a team of undergraduates from Sydney University. This is almost all of us -
With thanks to:
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