Team:SydneyUni Australia

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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
 
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{|align="justify"
 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
 
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|[[Image:SydneyUni_Australia_logo.png|200px|right|frame]]
 
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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|[[Image:SydneyUni_Australia_team.png|right|frame|Your team picture]]
 
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|align="center"|[[Team:SydneyUni_Australia | Team SydneyUni_Australia]]
 
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<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
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!align="center"|[[Team:SydneyUni_Australia/Safety|Safety]]
!align="center"|[[Team:SydneyUni_Australia/Safety|Safety]]
!align="center"|[[Team:SydneyUni_Australia/Attributions|Attributions]]
!align="center"|[[Team:SydneyUni_Australia/Attributions|Attributions]]
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{|align="justify"
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:SydneyUni_Australia_logo.png|200px|right|frame]]
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== '''Brief Project Description''' ==
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'''Background'''
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*1,2-dichloroethane (DCA) is part of a family of chlorinated hydrocarbons that are primarily derived from industrial solvents such as tetrachloroethene (PCE) and trichloroethene (TCE). Some of these organochlorines are toxic, carcinogenic and generally nasty.
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*DCA is a soluble and mobile contaminant of the groundwater in Botany Bay, Sydney, but also elsewhere around the world.
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*Conventional treatment involves significant costs, such as the pumping and heat-stripping of groundwater at Botany Bay. A biological alternative may be cheaper and more effective.
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*A suite of techniques (including sampling of contaminated sites, growth in bioreactors with selective conditions and protein engineering) have led to an understanding that there are two primary pathways of DCA-degradation (Fig. 1).
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'''Goals'''
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*Construct a BioBrick-compatible vector inspired by the broad host range vector, pBBR1MCS2.
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*Construct and compare two of the proposed pathways of DCA biodegradation.
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*Characterise the components of the DCA-degradation pathway for admission into the Registry of Standard Parts.
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*(If we get time!) Demonstrate the integration of our the pathway in the chromosome of Pseudomonas stutzeri via natural transformation and site-specific recombination.
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[[File:DCApathwaysHartman.jpg]]
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|align="center"|[[Team:SydneyUni_Australia | Team SydneyUni_Australia]]
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Revision as of 05:04, 16 July 2013


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions
You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
File:SydneyUni Australia logo.png
200px

Brief Project Description

Background

  • 1,2-dichloroethane (DCA) is part of a family of chlorinated hydrocarbons that are primarily derived from industrial solvents such as tetrachloroethene (PCE) and trichloroethene (TCE). Some of these organochlorines are toxic, carcinogenic and generally nasty.
  • DCA is a soluble and mobile contaminant of the groundwater in Botany Bay, Sydney, but also elsewhere around the world.
  • Conventional treatment involves significant costs, such as the pumping and heat-stripping of groundwater at Botany Bay. A biological alternative may be cheaper and more effective.
  • A suite of techniques (including sampling of contaminated sites, growth in bioreactors with selective conditions and protein engineering) have led to an understanding that there are two primary pathways of DCA-degradation (Fig. 1).

Goals

  • Construct a BioBrick-compatible vector inspired by the broad host range vector, pBBR1MCS2.
  • Construct and compare two of the proposed pathways of DCA biodegradation.
  • Characterise the components of the DCA-degradation pathway for admission into the Registry of Standard Parts.
  • (If we get time!) Demonstrate the integration of our the pathway in the chromosome of Pseudomonas stutzeri via natural transformation and site-specific recombination.


DCApathwaysHartman.jpg

Team SydneyUni_Australia
Retrieved from "http://2013.igem.org/Team:SydneyUni_Australia"