http://2013.igem.org/wiki/index.php?title=Team:TU-Delft/Timer_Plus_Sumo&feed=atom&action=historyTeam:TU-Delft/Timer Plus Sumo - Revision history2024-03-29T10:41:54ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:TU-Delft/Timer_Plus_Sumo&diff=291897&oldid=prevDimitra at 12:47, 4 October 20132013-10-04T12:47:14Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Figure 2: Simulation Results</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Figure 2: Simulation Results<ins class="diffchange diffchange-inline"><br></div></ins></p></div></td></tr>
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</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/Timer_Plus_Sumo&diff=291887&oldid=prevDimitra at 12:46, 4 October 20132013-10-04T12:46:42Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Figure 2: Simulation Results</p<del class="diffchange diffchange-inline">></div</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Figure 2: Simulation Results</p></div></td></tr>
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</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/Timer_Plus_Sumo&diff=291650&oldid=prevDimitra at 12:35, 4 October 20132013-10-04T12:35:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By this model we are finding answers to the following questions:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By this model we are finding answers to the following questions:</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>At what time point does the <del class="diffchange diffchange-inline">peptide_SUMO </del>start to be produced?</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>At what time point does the <ins class="diffchange diffchange-inline">peptide+SUMO </ins>start to be produced?</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>After how many minutes does the Ulp-1 cleave the peptide?</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>After how many minutes does the Ulp-1 cleave the peptide?</li></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the graph of Figure 2 the behavior of the circuit can be seen and the answers to the questions can be given:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the graph of Figure 2 the behavior of the circuit can be seen and the answers to the questions can be given:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ol> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ol> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>The peptide+SUMO is being produced at time point zero, reaching the maximum number of molecules, 3400 molecules, at time point <del class="diffchange diffchange-inline">62</del>.</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>The peptide+SUMO is being produced at time point zero, reaching the maximum number of molecules, 3400 molecules, at time point <ins class="diffchange diffchange-inline">70</ins>.</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>The cleaving of the SUMO can be observed from the start probably due to the fact that the promoter is leaky. After 80 minutes more Ulp-1 is produced, leading to an increased cleaving of the SUMO.</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>The cleaving of the SUMO can be observed from the start probably due to the fact that the promoter is leaky. After 80 minutes more Ulp-1 is produced, leading to an increased cleaving of the SUMO.</li></div></td></tr>
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</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/Timer_Plus_Sumo&diff=291631&oldid=prevDimitra at 12:34, 4 October 20132013-10-04T12:34:26Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>The peptide+SUMO is being produced at time point zero, reaching the maximum number of molecules, 3400 molecules, at time point 62.</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>The peptide+SUMO is being produced at time point zero, reaching the maximum number of molecules, 3400 molecules, at time point 62.</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>The cleaving of the SUMO can be observed from the start probably due to the fact that the promoter is leaky. <del class="diffchange diffchange-inline">Increased production rate of </del>Ulp-1 is <del class="diffchange diffchange-inline">observed after 80 minutes </del>leading to <del class="diffchange diffchange-inline">a </del></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>The cleaving of the SUMO can be observed from the start probably due to the fact that the promoter is leaky. <ins class="diffchange diffchange-inline">After 80 minutes more </ins>Ulp-1 is <ins class="diffchange diffchange-inline">produced, </ins>leading to <ins class="diffchange diffchange-inline">an increased cleaving of the SUMO.</ins></li></div></td></tr>
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</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/Timer_Plus_Sumo&diff=291563&oldid=prevDimitra at 12:30, 4 October 20132013-10-04T12:30:03Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2 align="center">Timer Plus Sumo</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2 align="center">Timer Plus Sumo</h2></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align="justify">In this section the system of Figure 1 is modeled. The structure of the timer has two repressing promoters (PcI and Ptet) and the input is the T7 promoter and the output is the protease Ulp-1. This Ulp-1 cleaves off the SUMO from the produced SUMO<del class="diffchange diffchange-inline">-</del>peptide.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align="justify">In this section the system of Figure 1 is modeled. The structure of the timer has two repressing promoters (PcI and Ptet) and the input is the T7 promoter and the output is the protease Ulp-1. This Ulp-1 cleaves off the SUMO from the produced SUMO<ins class="diffchange diffchange-inline">+</ins>peptide.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>By this model we are finding answers to the following <del class="diffchange diffchange-inline">question</del>:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>By this model we are finding answers to the following <ins class="diffchange diffchange-inline">questions</ins>:</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>After how many minutes does the <del class="diffchange diffchange-inline">SUMO </del>cleave the peptide?</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li>At what time point does the peptide_SUMO start to be produced?</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>After how many minutes does the <ins class="diffchange diffchange-inline">Ulp-1 </ins>cleave the peptide?</li></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h2 align="center">/<del class="diffchange diffchange-inline">ConclusionDiscussion</del></h2></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h2 align="center"><ins class="diffchange diffchange-inline">Conclusion</ins>/<ins class="diffchange diffchange-inline">Discussion</ins></h2></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In the graph of Figure 2 the <del class="diffchange diffchange-inline">answe</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In the graph of Figure 2 the <ins class="diffchange diffchange-inline">behavior of the circuit can be seen and the answers to the questions can be given:</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><ol> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li>The peptide+SUMO is being produced at time point zero, reaching the maximum number of molecules, 3400 molecules, at time point 62.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li>The cleaving of the SUMO can be observed from the start probably due to the fact that the promoter is leaky. Increased production rate of Ulp-1 is observed after 80 minutes leading to a </li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"></ol></ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">In the graph of Figure 2 the effect of the timer is clearly observed, at first the peptide+SUMO is produced, and after approximatly 80 minutes more Ulp is produced leading to an increased cleaving of the SUMO. Also note that there is cleaving of the SUMO already from the start, as the promoters are somewhat leaky. </del></div></td><td colspan="2"> </td></tr>
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</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/Timer_Plus_Sumo&diff=291299&oldid=prevDimitra at 12:16, 4 October 20132013-10-04T12:16:21Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">In the graph of Figure 2 the answe</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the graph of Figure 2 the effect of the timer is clearly observed, at first the peptide+SUMO is produced, and after approximatly 80 minutes more Ulp is produced leading to an increased cleaving of the SUMO. Also note that there is cleaving of the SUMO already from the start, as the promoters are somewhat leaky. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the graph of Figure 2 the effect of the timer is clearly observed, at first the peptide+SUMO is produced, and after approximatly 80 minutes more Ulp is produced leading to an increased cleaving of the SUMO. Also note that there is cleaving of the SUMO already from the start, as the promoters are somewhat leaky. </div></td></tr>
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</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/Timer_Plus_Sumo&diff=291278&oldid=prevDimitra at 12:14, 4 October 20132013-10-04T12:14:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By this model we are finding answers to the following question:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By this model we are finding answers to the following question:</div></td></tr>
</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/Timer_Plus_Sumo&diff=290915&oldid=prevDimitra at 11:56, 4 October 20132013-10-04T11:56:50Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By this model we are finding answers to the following question:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By this model we are finding answers to the following question:</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>After how many minutes does the SUMO cleave the peptide?</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>After how many minutes does the SUMO cleave the peptide?</li></div></td></tr>
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</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/Timer_Plus_Sumo&diff=290907&oldid=prevDimitra at 11:56, 4 October 20132013-10-04T11:56:24Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Figure 1: Circuit of the timer including sumo cleaving</p></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Figure 1: Circuit of the timer including sumo cleaving</p></div></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">By this model we are finding answers to the following question:</ins></div></td></tr>
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</table>Dimitrahttp://2013.igem.org/wiki/index.php?title=Team:TU-Delft/Timer_Plus_Sumo&diff=280079&oldid=prevJfkooijman at 17:47, 3 October 20132013-10-03T17:47:21Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Figure 2: Simulation Results</p></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Figure 2: Simulation Results</p></div></div></td></tr>
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</table>Jfkooijman