Team:TU-Eindhoven

From 2013.igem.org

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Latest revision as of 12:52, 26 October 2013

Welcome to the homepage of the TU Eindhoven 2013 iGEM team.

We will create bacteria that can detect tumors
and visualize them on an MRI scan.
Mission Statement of the TU/e iGEM 2013 Team

Our Project
Visualizing tumors using bacteria? Find out what our project is all about!

Learn more...
Wetlab
Take a look at the results of the broad range of experiments performed this year.

Learn more...
Drylab
Various simulations were carried out to support the wetlab experiments.

Learn more...

True. False. Inconclusive.

Which Facts are Synthetic?

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+===Welcome to the homepage of the TU Eindhoven 2013 iGEM team.===
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-==Project Description==
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-Our project focus on a relatively new form of {{:Team:TU-Eindhoven/Template:Tooltip | text=MRI | tooltip=Magnetic Resonance Imaging }}: {{:Team:TU-Eindhoven/Template:Tooltip | text=CEST | tooltip=Chemical Exchange Saturation Transfer }} imaging. CEST imaging proteins contain hydrogen atoms which can be used to create the same image quality as when conventional heavy metals are used.
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-We use Escherichia coli K-12 to create CEST proteins when the bacteria sense a {{:Team:TU-Eindhoven/Template:Tooltip | text=hypoxic | tooltip=Low oxygen saturation }} environment, thus working as a production factory and delivery system for the CEST MRI contrast agent. In order to achieve the production of our bacteria based CEST MRI contrast agent, we must create a series of diverse models focusing on the different components of the MRI contrast agent production. These models will range from the selection of the most adequate proteins for CEST MRI to the behaviour of the FNR promotor and the bacterial killing mechanism.
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-In the lab we attempt to produce the CEST MRI proteins, both aerobically and anaerobically. To Enable the anaerobic expression specialized promotors are designed to react to the changes in oxygen saturation and ultimately trigger the protein expression. Once the protein expression is successful either aerobically or anaerobically, these proteins are tested for the quality of their contrast in an MRI machine.
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+}(document, 'script', 'facebook-jssdk'));</script><div class="fb-like" data-href="http://www.facebook.com/iGEMEindhoven" data-send="false" data-layout="box_count" data-width="450" data-show-faces="false" style="height: 61px; margin-bottom: -61px; width: 50px; overflow: hidden;"></div></div><div style="float: right;"><img src="</html>{{filepath: projectOverview.jpg | nowiki}}<html>" style="width: 300px;" /></div></div></div>
-Based on the principle of our project, we propose two applications, tumor CEST MR Imaging and tracking of bacteria in bacterial infections studies. It is well known that tumors present a hypoxic environment, therefore our bacteria can be injected into bloodstream to travel into the tumor region. Once in the tumor region, the hypoxic conditions of the area will trigger the production of the CEST proteins by the E. coli. generating the required CEST contrast agent for MRI. This will ensure that MRI contrast is created where tumors are present, and also provides a good means of tumor targeting for drug delivery systems in the future. Once the CEST MRI images have been taken, the bacteria will be killed and eliminated from the body using our killing mechanism. In order to be injected into the bloodstream the bacteria should be modified once more to avoid activating an immune response. Similarly, our production and delivery system can be used for tracking bacteria in bacterial infection studies.
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 {{:Team:TU-Eindhoven/Template:FrontThumbnail | image=TU-Eindhoven_Images_MRIPromo.jpg | link=/Team:TU-Eindhoven/Wetlab | heading=Wetlab | text=Take a look at the results of the broad range of experiments performed this year. }}
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+<html><div class="thumbnail" style="background: url('https://static.igem.org/mediawiki/2013/2/2d/TU-Eindhoven_Images_Header4.jpg') repeat-x center top !important; height: 150px;"><center><div style="font-size: 48pt; line-height: 48pt; text-algin: center; margin-top: 20px; font-weight: bold; color: #555555;">True. False. Inconclusive.</div><div style="font-size: 48pt; line-height: 48pt; text-algin: center; margin-top: -48pt; font-weight: bold; color: #555555;"><span id="tue-sf-true" style="color: #008000; opacity: 0.0;">True.</span> <span id="tue-sf-false" style="color: #FF0000; opacity: 0.0;">False.</span> <span id="tue-sf-undecided" style="color: #0000FF; opacity: 0.0;">Inconclusive.</span></div><br /><a class="btn btn-info" target="_blank" href="http://syntheticfacts.tue.nl">Which Facts are Synthetic?</a></center></div></html>
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