Team:TU-Eindhoven/Notebook

From 2013.igem.org

(Difference between revisions)
Line 36: Line 36:
* Researching about the promoters
* Researching about the promoters
* Researching about the possible proteins for CEST imaging
* Researching about the possible proteins for CEST imaging
 +
''Drylab''
''Drylab''
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''Wetlab''
''Wetlab''
* Researching and defining the killing mechanism
* Researching and defining the killing mechanism
 +
''Drylab''
''Drylab''
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* Regular team meeting
* Regular team meeting
* Looking for sponsors
* Looking for sponsors
 +
''Wetlab''
''Wetlab''
* Defining the needed materials
* Defining the needed materials
* Defining the needed equipment
* Defining the needed equipment
 +
''Drylab''
''Drylab''
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* Regular team meeting
* Regular team meeting
* Looking for sponsors
* Looking for sponsors
 +
''Wetlab''
''Wetlab''
* Locating, booking and learning how to use the necessary equipment
* Locating, booking and learning how to use the necessary equipment
 +
''Drylab''
''Drylab''
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* Regular team meeting
* Regular team meeting
* Looking for sponsors
* Looking for sponsors
 +
''Wetlab''
''Wetlab''
* Locating, booking and learning how to use the necessary equipment
* Locating, booking and learning how to use the necessary equipment
 +
''Drylab''
''Drylab''
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* Collaborating with Purdue iGEM team
* Collaborating with Purdue iGEM team
* Planning month activities
* Planning month activities
 +
''Wetlab''
''Wetlab''
* General construct design
* General construct design
 +
''Drylab''
''Drylab''
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* Regular team meeting
* Regular team meeting
* Looking for sponsors
* Looking for sponsors
 +
''Wetlab''
''Wetlab''
* Protein design
* Protein design
 +
''Drylab''
''Drylab''
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* Regular team meeting
* Regular team meeting
* Looking for sponsors
* Looking for sponsors
 +
''Wetlab''
''Wetlab''
* Final design of the constructs
* Final design of the constructs
 +
''Drylab''
''Drylab''
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* Regular team meeting
* Regular team meeting
* Looking for sponsors
* Looking for sponsors
 +
''Wetlab''
''Wetlab''
* Ordering the constructs
* Ordering the constructs
* Working on the protocols
* Working on the protocols
 +
''Drylab''
''Drylab''
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* Looking for sponsors
* Looking for sponsors
* Planning month activities
* Planning month activities
 +
''Wetlab''
''Wetlab''
* Finalizing the protocols
* Finalizing the protocols
* Planning lab schedule
* Planning lab schedule
 +
''Drylab''
''Drylab''
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* Looking for sponsors
* Looking for sponsors
* Collaborating with Purdue iGEM team
* Collaborating with Purdue iGEM team
 +
''Drylab''
''Drylab''
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* Looking for sponsors
* Looking for sponsors
* Collaborating with Purdue iGEM team
* Collaborating with Purdue iGEM team
 +
''Wetlab''
''Wetlab''
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* DNA retention from cultures
* DNA retention from cultures
* Nano-drop tests
* Nano-drop tests
 +
''Drylab''
''Drylab''
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* Looking for sponsors
* Looking for sponsors
 +
''Wetlab''
* Creating agar plates
* Creating agar plates
* Transforming DNA
* Transforming DNA

Revision as of 15:46, 6 August 2013

In this section you can find a small description of all the work we have done so far organized by the category it belongs to.


Feb-Apr

Feb-Mar

  • Background research


Mar-Apr

  • Selection of topic
  • Further research
  • Selection of project

Apr-May

  • Further research


May

1-5 May
General Activities

  • Regular team meeting
  • Looking for sponsors
  • Month planning


Wetlab

  • Researching about the promoters
  • Researching about the possible proteins for CEST imaging


Drylab

  • Defining what needs to be modeled and what type of model is the most appropriate for it

6-12 May
General Activities

  • Regular team meeting
  • Looking for sponsors


Wetlab

  • Researching and defining the killing mechanism


Drylab

  • Starting the Molecular Dynamics model for the proteins selection.

13-19 May
General Activities

  • Regular team meeting
  • Looking for sponsors


Wetlab

  • Defining the needed materials
  • Defining the needed equipment


Drylab

  • Continuing the Molecular Dynamics model for the proteins selection.

20-26 May
General Activities

  • Regular team meeting
  • Looking for sponsors


Wetlab

  • Locating, booking and learning how to use the necessary equipment


Drylab

  • Analyzing the results from the Molecular Dynamics model for the proteins selection and identifying the most useful proteins for CEST imaging
  • Researching and defining the needed parameters for the analysis of the decoy sites through a stochastic simulations model

27-31 May
General Activities

  • Regular team meeting
  • Looking for sponsors


Wetlab

  • Locating, booking and learning how to use the necessary equipment


Drylab

  • Starting the stochastic simulations for the analysis of the decoy sites

June

3-9 June
General Activities

  • Regular team meeting
  • Looking for sponsors
  • Collaborating with Purdue iGEM team
  • Planning month activities


Wetlab

  • General construct design


Drylab

  • Continuing the stochastic simulations for the analysis of the decoy sites

10-16 June
General Activities

  • Regular team meeting
  • Looking for sponsors


Wetlab

  • Protein design


Drylab

  • Continuing the stochastic simulations for the analysis of the decoy sites

17-23 June
General Activities

  • Regular team meeting
  • Looking for sponsors


Wetlab

  • Final design of the constructs


Drylab

  • Continuing the stochastic simulations for the analysis of the decoy sites

24-30 June
General Activities

  • Regular team meeting
  • Looking for sponsors


Wetlab

  • Ordering the constructs
  • Working on the protocols


Drylab

  • Analyzing the results from the stochastic simulations for the analysis of the decoy sites

July

1-7 July
General Activities

  • Regular team meeting
  • Looking for sponsors
  • Planning month activities


Wetlab

  • Finalizing the protocols
  • Planning lab schedule


Drylab

  • Starting the ODE model for the analysis of the FNR promoter

8-14 July
General Activities

  • Regular team meeting
  • Looking for sponsors
  • Collaborating with Purdue iGEM team


Drylab

  • Continuing the ODE model for the analysis of the FNR promoter

15-21 July

22-28 July
General Activities

  • Regular team meetings
  • Looking for sponsors
  • Collaborating with Purdue iGEM team


Wetlab

  • Creating agar plates
  • Transforming DNA
  • Plating bacteria
  • Making LB medium
  • Creating small cultures
  • DNA retention from cultures
  • Nano-drop tests


Drylab

  • Analyzing the results from the ODE model for the analysis of the FNR promoter

29-31 July
General Activities

  • Regular team meetings
  • Looking for sponsors

Wetlab

  • Creating agar plates
  • Transforming DNA
  • Plating bacteria
  • Making LB medium
  • Creating small cultures
  • DNA retention from cultures
  • Nano-drop tests

Drylab

  • Merging the stochastic simulations model for the decoy sites with the ODE model for the FNR promoter

August

1-4 August
2013

  • ...

5-11 August
2013

  • ...

12-18 August
2013

  • ...

19-25 August
2013

  • ...

26-31 August
2013

  • ...

September

2-8 September
2013

  • ...

9-15 September
2013

  • ...

16-22 September
2013

  • ...

23-29 September
2013

  • ...