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Team:TU-Eindhoven/Notebook
From 2013.igem.org
In this section you can find a small description of all the work we have done so far organized by the category it belongs to.
Feb-Apr
- Background research
- Selection of topic
- Further research
- Selection of project
- Further research
May
General Activities
- Regular team meeting
- Looking for sponsors
- Month planning
Wetlab
- Researching about the promoters
- Researching about the possible proteins for CEST imaging
Drylab
- Defining what needs to be modeled and what type of model is the most appropriate for it
General Activities
- Regular team meeting
- Looking for sponsors
Wetlab
- Researching and defining the killing mechanism
Drylab
- Starting the Molecular Dynamics model for the proteins selection.
General Activities
- Regular team meeting
- Looking for sponsors
Wetlab
- Defining the needed materials
- Defining the needed equipment
Drylab
- Continuing the Molecular Dynamics model for the proteins selection.
General Activities
- Regular team meeting
- Looking for sponsors
Wetlab
- Locating, booking and learning how to use the necessary equipment
Drylab
- Analyzing the results from the Molecular Dynamics model for the proteins selection and identifying the most useful proteins for CEST imaging
- Researching and defining the needed parameters for the analysis of the decoy sites through a stochastic simulations model
General Activities
- Regular team meeting
- Looking for sponsors
Wetlab
- Locating, booking and learning how to use the necessary equipment
Drylab
- Starting the stochastic simulations for the analysis of the decoy sites
June
General Activities
- Regular team meeting
- Looking for sponsors
- Collaborating with Purdue iGEM team
- Planning month activities
Wetlab
- General construct design
Drylab
- Continuing the stochastic simulations for the analysis of the decoy sites
General Activities
- Regular team meeting
- Looking for sponsors
Wetlab
- Protein design
Drylab
- Continuing the stochastic simulations for the analysis of the decoy sites
General Activities
- Regular team meeting
- Looking for sponsors
Wetlab
- Final design of the constructs
Drylab
- Continuing the stochastic simulations for the analysis of the decoy sites
General Activities
- Regular team meeting
- Looking for sponsors
Wetlab
- Ordering the constructs
- Working on the protocols
Drylab
- Analyzing the results from the stochastic simulations for the analysis of the decoy sites
July
General Activities
- Regular team meeting
- Looking for sponsors
- Planning month activities
Wetlab
- Finalizing the protocols
- Planning lab schedule
Drylab
- Starting the ODE model for the analysis of the FNR promoter
General Activities
- Regular team meeting
- Looking for sponsors
- Collaborating with Purdue iGEM team
Drylab
- Continuing the ODE model for the analysis of the FNR promoter
General Activities
- Regular team meetings
- Looking for sponsors
- Collaborating with Purdue iGEM team
Wetlab
- Creating agar plates
- Transforming DNA
- Plating bacteria
- Making LB medium
- Creating small cultures
- DNA retention from cultures
- Nano-drop tests
Drylab
- Analyzing the results from the ODE model for the analysis of the FNR promoter
General Activities
- Regular team meetings
- Looking for sponsors
- Creating agar plates
- Transforming DNA
- Plating bacteria
- Making LB medium
- Creating small cultures
- DNA retention from cultures
- Nano-drop tests
Drylab
- Merging the stochastic simulations model for the decoy sites with the ODE model for the FNR promoter
August
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September
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