Labwork Description

Within the scope of the 2013 iGEM competition our goal is to create tumor targeting bacteria which, upon the sensing of a tumor, would produce proteins that could be used for the imaging thereof. To achieve this, it is necessary to design and develop a system that targets and reacts to tumors, and is able to produce the proteins that enable the tumor imaging. In order to develop such a system, we first defined the DNA CONSTRUCTS that constitute the basis of all our lab work. To ensure that the best possible protein was used for the tumor imaging it was decided to design and produce multiple different proteins. Once we generated our CEST contrast agent device, we tested the imaging qualities of the CEST contrast agent obtained by producing different proteins. The latter was done with an MRI SCANNER. We also evaluated the ability of our device to generate the contrast agent under hypoxic conditions by performing an ANAEROBIC TESTING of the FNR Promoter.

The complete procedures and protocols that we performed within our lab work can be found on the LAB JORUNAL.