Team:TU-Munich/Project/Overview

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Project Overview

In the 2013 competition the TU Munich iGEM team has developed a transgenic moss filter which is capable to reduct the the contamination of aquatic ecosystems with xenobiotics. The topic of remediation using transgenic organisms is present in every year of iGEM and we want to take this idea to the next level. For this reason we decided to use a photoautotrophic chassis which we enabled for the iGEM competition. The following section gives an overview over the theoretic background of our project.


Phytoremediation

Phytoremediation describes the treatment of environmental problems through the use of plants. Here we identified problematic substances and described basic principles (Read more).

PHyscomitrella

As next point we introduce Physcomitrella patens as a nearly new chassis to iGEM. We describe advantages and possiblilities and describe our expression strategy (Read more).

Localisation

As the principle of action for the effectors responsible for the Phytoremediation are very different it is necessary to have a expression system which is able to accomplish cytosolic, secreted, receptor bound localisation of proteins. Additionally the SypTag system was implemented to get the system ready for the future when several effectors have to be expressed in parallel in one moss strain.

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