Team:Tuebingen/Notebook/Protocols/sob-medium
From 2013.igem.org
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<h3>Reagents</h3> | <h3>Reagents</h3> | ||
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<td style="text-align: center">20.0 g</td> | <td style="text-align: center">20.0 g</td> | ||
- | <td> | + | <td>Trypton</td> |
</tr> | </tr> | ||
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<td style="text-align: center">5.0 g</td> | <td style="text-align: center">5.0 g</td> | ||
- | <td> | + | <td>Yeast extract</td> |
</tr> | </tr> | ||
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<td style="text-align: center">0.5 g</td> | <td style="text-align: center">0.5 g</td> | ||
- | <td> | + | <td>NaCl</td> |
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<td style="text-align: center">10 mL</td> | <td style="text-align: center">10 mL</td> | ||
- | <td> | + | <td>KCl (c = 250 mM)</td> |
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+ | <p> </p> | ||
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+ | <h3>Procedure</h3> | ||
+ | <p>In an autoclavable flask, dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. After autoclaving add 5 mL MgCL2 (c = 2 M).</p> | ||
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+ | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> | ||
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Latest revision as of 12:08, 4 October 2013
SOB Medium
Reagents
20.0 g | Trypton |
5.0 g | Yeast extract |
0.5 g | NaCl |
10 mL | KCl (c = 250 mM) |
Procedure
In an autoclavable flask, dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. After autoclaving add 5 mL MgCL2 (c = 2 M).
Back to Protocols