http://2013.igem.org/wiki/index.php?title=Team:UCL/Notebook/August&feed=atom&action=historyTeam:UCL/Notebook/August - Revision history2024-03-28T21:32:08ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:UCL/Notebook/August&diff=315910&oldid=prevNaruto at 03:15, 5 October 20132013-10-05T03:15:31Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Stjohn designed the linkers for the Mammalian Oxidative Stress Inducible Promoter.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Stjohn designed the linkers for the Mammalian Oxidative Stress Inducible Promoter.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The team met to discuss fundraising ideas <del class="diffchange diffchange-inline">somehow making use of [kickstarter</del>.<del class="diffchange diffchange-inline">com]. A starting </del>idea: brain-with-plaques-for-sale.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The team met to discuss fundraising ideas <ins class="diffchange diffchange-inline">such as Kickstarter</ins>. <ins class="diffchange diffchange-inline">Starting </ins>idea: brain-with-plaques-for-sale.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="body_text"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="body_text"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">We came up with the idea </del>of a Memory Lane, where people could upload <del class="diffchange diffchange-inline">a photo of one of </del>their memories <del class="diffchange diffchange-inline">and write </del>a <del class="diffchange diffchange-inline">small description about it</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The concept </ins>of a Memory Lane, where people could upload their memories <ins class="diffchange diffchange-inline">onto </ins>a <ins class="diffchange diffchange-inline">page within the wiki, was discussed</ins>. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">The team worked on </del>the <del class="diffchange diffchange-inline">abstract which must be uploaded shortly on </del>wiki <del class="diffchange diffchange-inline">as the deadline is on the 9th</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Team abstracts were written and uploaded onto </ins>the wiki. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Darren <del class="diffchange diffchange-inline">gave us a visit at </del>the lab to check <del class="diffchange diffchange-inline">if everything is O.K. with our work and enthusiasm</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Dr </ins>Darren <ins class="diffchange diffchange-inline">Nesbeth visted </ins>the lab <ins class="diffchange diffchange-inline">on numerous occasions </ins>to check <ins class="diffchange diffchange-inline">project progress</ins>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The requested batch of biobricks arrived as glycerol stocks.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The requested batch of biobricks arrived as glycerol stocks.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="body_text"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="body_text"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The team discussed <del class="diffchange diffchange-inline">about </del>Kickstarter <del class="diffchange diffchange-inline">crowdfunding and planned to launch the Memory Lane/Map thing WITHOUT getting people to pay</del>. <del class="diffchange diffchange-inline">We will get people </del>to <del class="diffchange diffchange-inline">upload their best memories in different forms and potentially do some beautiful art with it like </del>the Memory Palace <del class="diffchange diffchange-inline">FYi suggested</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The team <ins class="diffchange diffchange-inline">further </ins>discussed <ins class="diffchange diffchange-inline">crowdfunding via </ins>Kickstarter. <ins class="diffchange diffchange-inline">Beautiful pieces of art are </ins>to <ins class="diffchange diffchange-inline">be generated by FYi for </ins>the Memory Palace. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="minor_title">12th August</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="minor_title">12th August</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">We had a </del>strategy <del class="diffchange diffchange-inline">chat at </del>the lab with Darren. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">A </ins>strategy <ins class="diffchange diffchange-inline">meeting was held in </ins>the lab with <ins class="diffchange diffchange-inline">Dr </ins>Darren <ins class="diffchange diffchange-inline">Nesbeth</ins>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="body_text"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="body_text"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>FYi drawn the wiki background for the diary section. She also made the illustrations for the T-shirts.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>FYi drawn the wiki background for the diary section. She also made the illustrations for the T-shirts.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The team also debated on the wiki design and a consensus was reached regarding the site map, default banner<del class="diffchange diffchange-inline">, </del>logo.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The team also debated on the wiki design and a consensus was reached regarding the site map, default banner <ins class="diffchange diffchange-inline">and </ins>logo.</div></td></tr>
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</table>Narutohttp://2013.igem.org/wiki/index.php?title=Team:UCL/Notebook/August&diff=314779&oldid=prevAlexBates at 02:36, 5 October 20132013-10-05T02:36:48Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We had a strategy chat at the lab with Darren. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We had a strategy chat at the lab with Darren. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class="body_text"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>FYi drawn the wiki background for the diary section. She also made the illustrations for the T-shirts.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>FYi drawn the wiki background for the diary section. She also made the illustrations for the T-shirts.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></p></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The team also debated on the wiki design and a consensus was reached regarding the site map, default banner, logo.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The team also debated on the wiki design and a consensus was reached regarding the site map, default banner, logo.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class="body_text"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In 'Memory Lane', we are going to ask people to 'leave one strong memory' on one page whether in text or pictures. These will be done anonymously but they will leave their emails with us so they will be notified when the 'compilation' is up. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In 'Memory Lane', we are going to ask people to 'leave one strong memory' on one page whether in text or pictures. These will be done anonymously but they will leave their emails with us so they will be notified when the 'compilation' is up. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The website came to life today!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The website came to life today!</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Alex and Oran came up with the idea of a Creative writing competition. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Alex and Oran came up with the idea of a Creative writing competition. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class="body_text"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>FYi, Robin, Alex and Stjohn and Oran focused on wiki building for the weeks to come while the rest of the team worked in the Bacterial Labs.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>FYi, Robin, Alex and Stjohn and Oran focused on wiki building for the weeks to come while the rest of the team worked in the Bacterial Labs.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The advertisement for the competition was written and the competition was launched. More details about the outcome can be found on the ‘Competition’ subsection.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The advertisement for the competition was written and the competition was launched. More details about the outcome can be found on the ‘Competition’ subsection.</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class="body_text"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Met the Westminister team to discuss about the potential modelling collaboration. It was a nice gathering.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Met the Westminister team to discuss about the potential modelling collaboration. It was a nice gathering.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="minor_title">16th August</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="minor_title">16th August</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="<ins class="diffchange diffchange-inline">body_text</ins>"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Alex finished the essay on Neuroethics on which he has dedicated around 2 weeks of research.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p class="<del class="diffchange diffchange-inline">minor_title</del>"<del class="diffchange diffchange-inline">>17th August</p</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><p class="minor_title">18th August</del></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="minor_title">19th August</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="minor_title">19th August</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Alex advertised the writing competition on prizemagic.co.uk.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Alex advertised the writing competition on prizemagic.co.uk.</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class="body_text"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Stjohn released a new set of rules for managing wiki content in order to make work easier before the wiki freeze.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Stjohn released a new set of rules for managing wiki content in order to make work easier before the wiki freeze.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The actual work on the main poster on the frontal page started. FYi produced the first sketch and the team gave feedback.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The actual work on the main poster on the frontal page started. FYi produced the first sketch and the team gave feedback.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class="body_text"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The members’ Profiles are ready to be uploaded on wiki!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The members’ Profiles are ready to be uploaded on wiki!</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 177:</td>
<td colspan="2" class="diff-lineno">Line 180:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The lab was closed in the morning, however in the afternoon the Bacteria Team prepared selective plates and selective media in order to culture the last arrived biobricks from the HQ. Darren assisted us.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The lab was closed in the morning, however in the afternoon the Bacteria Team prepared selective plates and selective media in order to culture the last arrived biobricks from the HQ. Darren assisted us.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class="body_text"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The linkers designed by Stjohn: IGM Ox L1, L2, L3, L4 as well primers for cmv promoter were ordered.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The linkers designed by Stjohn: IGM Ox L1, L2, L3, L4 as well primers for cmv promoter were ordered.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 184:</td>
<td colspan="2" class="diff-lineno">Line 188:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The first Creative Competition Entry! Yey! Thank you!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The first Creative Competition Entry! Yey! Thank you!</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class="body_text"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The atmosphere in the Bacterial Lab became slightly more cheerful. The amplification of zeocin from the 2 types of ordered primers was successful as well as the digestion of K812014 and pSB1C3 and pSB1A3. We decided to use the zec bb F,R primers for the further amplification of zeocin. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The atmosphere in the Bacterial Lab became slightly more cheerful. The amplification of zeocin from the 2 types of ordered primers was successful as well as the digestion of K812014 and pSB1C3 and pSB1A3. We decided to use the zec bb F,R primers for the further amplification of zeocin. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The <del class="diffchange diffchange-inline">Zeo </del>kill curve was derived, a concentration of 150 ug/ml was used.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p class="body_text"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The <ins class="diffchange diffchange-inline">Zeocin </ins>kill curve was derived, a concentration of 150 ug/ml was used.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 193:</td>
<td colspan="2" class="diff-lineno">Line 199:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The main poster for the front page was finalised. Well done FYi!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The main poster for the front page was finalised. Well done FYi!</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class="body_text"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>New submissions for the Creative writing! </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>New submissions for the Creative writing! </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Lonza confirmed a sponsorship of £1, 207. Happy Happy Joy Joy! Well done Weiling!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Lonza confirmed a sponsorship of £1, 207. Happy Happy Joy Joy! Well done Weiling!</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 202:</td>
<td colspan="2" class="diff-lineno">Line 209:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The lab was closed today hence we all focused on the wiki content.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The lab was closed today hence we all focused on the wiki content.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class="body_text"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The front page poster background - wasteland was completed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The front page poster background - wasteland was completed.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 207:</td>
<td colspan="2" class="diff-lineno">Line 215:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="minor_title">27th August</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="minor_title">27th August</p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class="body_text"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Weiling emailed Geneious and Eppendorf with regards to Sponsorship.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></p></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="minor_title">28th August</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="minor_title">28th August</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 212:</td>
<td colspan="2" class="diff-lineno">Line 223:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Biosafety forms were filled in as necessary. These must be signed by Darren before the 30th.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Biosafety forms were filled in as necessary. These must be signed by Darren before the 30th.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class="body_text"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We met Darren at 4 pm in the lab to discuss about the biobrick processing.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We met Darren at 4 pm in the lab to discuss about the biobrick processing.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 219:</td>
<td colspan="2" class="diff-lineno">Line 231:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We considered the strategy to deal with the linker region. First step is to achieve the annealing of the oligonucleotides making up this linker. We're still waiting for these sequences.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We considered the strategy to deal with the linker region. First step is to achieve the annealing of the oligonucleotides making up this linker. We're still waiting for these sequences.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class="body_text"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Agreed on the final design of the T-shirts. We're aiming to order them as soon as possible.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Agreed on the final design of the T-shirts. We're aiming to order them as soon as possible.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 226:</td>
<td colspan="2" class="diff-lineno">Line 239:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We uploaded the first samples of memories on the Memory Lane page.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We uploaded the first samples of memories on the Memory Lane page.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>AlexBateshttp://2013.igem.org/wiki/index.php?title=Team:UCL/Notebook/August&diff=311001&oldid=prevNaruto: Created page with "<html> <head> <script type="text/javascript" src="https://2013.igem.org/Team:UCL/static/head.js?action=raw&ctype=text/javascript"> </script> <link rel="stylesheet" type="text..."2013-10-05T00:42:09Z<p>Created page with "<html> <head> <script type="text/javascript" src="https://2013.igem.org/Team:UCL/static/head.js?action=raw&ctype=text/javascript"> </script> <link rel="stylesheet" type="text..."</p>
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<p class="body_text"><a href="https://2013.igem.org/Team:UCL/Notebook/January">January</a> | <a href="https://2013.igem.org/Team:UCL/Notebook/February">February</a> | <a href="https://2013.igem.org/Team:UCL/Notebook/March">March</a> | <a href="https://2013.igem.org/Team:UCL/Notebook/April">April</a> | <a href="https://2013.igem.org/Team:UCL/Notebook/May">May</a> | <a href="https://2013.igem.org/Team:UCL/Notebook/June">June</a> | <a href="https://2013.igem.org/Team:UCL/Notebook/July">July</a> | <a href="https://2013.igem.org/Team:UCL/Notebook/August">August</a> | <a href="https://2013.igem.org/Team:UCL/Notebook/September">September</a> | <a href="https://2013.igem.org/Team:UCL/Notebook/October">October</a> <br />
</p> <br />
</div><br />
<br />
<p class="major_title">August</p><br />
<div class="full_row"><br />
<div class="gap"><br />
</div><br />
<p class="minor_title">1st August</p><br />
<p class="body_text"><br />
Bacterial lab had good results today in the preparation of a new stock of competent cells.<br />
In the evening we celebrated the success of the speed debate.<br />
</p><br />
<p class="minor_title">2nd August</p><br />
<p class="body_text"><br />
Stjohn designed the linkers for the Mammalian Oxidative Stress Inducible Promoter.<br />
The team met to discuss fundraising ideas somehow making use of [kickstarter.com]. A starting idea: brain-with-plaques-for-sale.<br />
</p><br />
<p class="body_text"><br />
We came up with the idea of a Memory Lane, where people could upload a photo of one of their memories and write a small description about it.<br />
</p><br />
<p class="body_text"><br />
Alex suggested a collaboration with Westminster iGEM team regarding the speed debate idea.<br />
</p><br />
<br />
<p class="minor_title">5th August</p><br />
<p class="body_text"><br />
Snapshots of the team members were taken!<br />
</p><br />
<p class="body_text"><br />
The team worked on the abstract which must be uploaded shortly on wiki as the deadline is on the 9th.<br />
</p><br />
<p class="body_text"><br />
Alex contacted the Imperial iGEM team regarding an eventual collaboration. <br />
</p><br />
<br />
<p class="minor_title">6th August</p><br />
<p class="body_text"><br />
Rob invited the team at 12 noon in the Anatomy Hub to discuss about the wiki design in order to make sure that all the ideas about this matter are taken into account.<br />
</p><br />
<br />
<p class="minor_title">7th August</p><br />
<p class="body_text"><br />
Barbeque evening, venue Wilkins Roof Garden!<br />
</p><br />
<p class="body_text"><br />
Prof. Eli Keshavarz-Moore was our guest and at 3 pm we also had the chance to present our project. (venue: Malet Place Engineering LT 1.03)<br />
</p><br />
<br />
<p class="minor_title">8th August</p><br />
<p class="body_text"><br />
The team discussed about the work on zeocin,pA-f1-Zec biobrick, which will indeed be an improvement of BBa_J176124 because:<br />
</p><br />
<p class="body_text"><br />
i) it gives most of the functionality of BBa_J176124 but is compatible with standard assembly<br />
</p><br />
<p class="body_text"><br />
ii) it allows people to simply insert a PROMOTER-ORF fragment upstream of a pA to give an expression cassette for the ORF of interest, and a ZEC to select stable transfectants. <br />
</p><br />
<br />
<p class="minor_title">9th August</p><br />
<p class="body_text"><br />
Project description is up on Wiki!<br />
</p><br />
<p class="body_text"><br />
Darren gave us a visit at the lab to check if everything is O.K. with our work and enthusiasm.<br />
The requested batch of biobricks arrived as glycerol stocks.<br />
</p><br />
<p class="body_text"><br />
The team discussed about Kickstarter crowdfunding and planned to launch the Memory Lane/Map thing WITHOUT getting people to pay. We will get people to upload their best memories in different forms and potentially do some beautiful art with it like the Memory Palace FYi suggested. <br />
</p><br />
<br />
<p class="minor_title">12th August</p><br />
<p class="body_text"><br />
We had a strategy chat at the lab with Darren. <br />
</p><br />
FYi drawn the wiki background for the diary section. She also made the illustrations for the T-shirts.<br />
</p><br />
The team also debated on the wiki design and a consensus was reached regarding the site map, default banner, logo.<br />
</p><br />
In 'Memory Lane', we are going to ask people to 'leave one strong memory' on one page whether in text or pictures. These will be done anonymously but they will leave their emails with us so they will be notified when the 'compilation' is up. <br />
</p><br />
The website came to life today!<br />
</p><br />
<br />
<br />
<p class="minor_title">13th August</p><br />
<p class="body_text"><br />
Alex and Oran came up with the idea of a Creative writing competition. <br />
</p><br />
FYi, Robin, Alex and Stjohn and Oran focused on wiki building for the weeks to come while the rest of the team worked in the Bacterial Labs.<br />
</p><br />
<br />
<br />
<p class="minor_title">14th August</p><br />
<p class="body_text"><br />
The advertisement for the competition was written and the competition was launched. More details about the outcome can be found on the ‘Competition’ subsection.<br />
</p><br />
Met the Westminister team to discuss about the potential modelling collaboration. It was a nice gathering.<br />
</p><br />
<br />
<p class="minor_title">15th August</p><br />
<p class="body_text"><br />
Continued intensively planning and brainstorming for the design of our wiki, especially on the front page design. <br />
</p><br />
<br />
<p class="minor_title">16th August</p><br />
<br />
<br />
<br />
<p class="minor_title">17th August</p><br />
<br />
<p class="minor_title">18th August</p><br />
<br />
<p class="minor_title">19th August</p><br />
<p class="body_text"><br />
Alex advertised the writing competition on prizemagic.co.uk.<br />
</p><br />
Stjohn released a new set of rules for managing wiki content in order to make work easier before the wiki freeze.<br />
</p><br />
<br />
<p class="minor_title">20th August</p><br />
<p class="body_text"><br />
The actual work on the main poster on the frontal page started. FYi produced the first sketch and the team gave feedback.<br />
</p><br />
The members’ Profiles are ready to be uploaded on wiki!<br />
</p><br />
<br />
<p class="minor_title">21th August</p><br />
<p class="body_text"><br />
The lab was closed in the morning, however in the afternoon the Bacteria Team prepared selective plates and selective media in order to culture the last arrived biobricks from the HQ. Darren assisted us.<br />
</p><br />
The linkers designed by Stjohn: IGM Ox L1, L2, L3, L4 as well primers for cmv promoter were ordered.<br />
</p><br />
<br />
<p class="minor_title">22th August</p><br />
<p class="body_text"><br />
The first Creative Competition Entry! Yey! Thank you!<br />
</p><br />
The atmosphere in the Bacterial Lab became slightly more cheerful. The amplification of zeocin from the 2 types of ordered primers was successful as well as the digestion of K812014 and pSB1C3 and pSB1A3. We decided to use the zec bb F,R primers for the further amplification of zeocin. <br />
</p><br />
The Zeo kill curve was derived, a concentration of 150 ug/ml was used.<br />
</p><br />
<br />
<p class="minor_title">23th August</p><br />
<p class="body_text"><br />
The main poster for the front page was finalised. Well done FYi!<br />
</p><br />
New submissions for the Creative writing! <br />
Lonza confirmed a sponsorship of £1, 207. Happy Happy Joy Joy! Well done Weiling!<br />
</p><br />
<br />
<br />
<p class="minor_title">26th August</p><br />
<p class="body_text"><br />
The lab was closed today hence we all focused on the wiki content.<br />
</p><br />
The front page poster background - wasteland was completed.<br />
</p><br />
<br />
<br />
<p class="minor_title">27th August</p><br />
<br />
<p class="minor_title">28th August</p><br />
<p class="body_text"><br />
The Biosafety forms were filled in as necessary. These must be signed by Darren before the 30th.<br />
</p><br />
We met Darren at 4 pm in the lab to discuss about the biobrick processing.<br />
</p><br />
<br />
<p class="minor_title">29th August</p><br />
<p class="body_text"><br />
We considered the strategy to deal with the linker region. First step is to achieve the annealing of the oligonucleotides making up this linker. We're still waiting for these sequences.<br />
</p><br />
Agreed on the final design of the T-shirts. We're aiming to order them as soon as possible.<br />
</p><br />
<br />
<p class="minor_title">30th August</p><br />
<p class="body_text"><br />
We uploaded the first samples of memories on the Memory Lane page.<br />
</p><br />
</div><br />
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