Team:UCSF/Project/Background2
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- | <p2> As a means <a href="https://2013.igem.org/Team:UCSF/Project/Conjugation/Design" >to introduce our CRISPRi system into a microbial community we've opted to utilize conjugation </a> - a naturally occurring mechanism bacteria use to transfer DNA. By utilizing this mechanism, we are able to target specific strains of bacteria and affect gene expression. This will have a potential for future applications that require targeting individual strains in a bacterial community. </p2></div> | + | <p2> As a means <a href="https://2013.igem.org/Team:UCSF/Project/Conjugation/Design" > to introduce our CRISPRi system into a microbial community we've opted to utilize conjugation </a> - a naturally occurring mechanism bacteria use to transfer DNA. By utilizing this mechanism, we are able to target specific strains of bacteria and affect gene expression. This will have a potential for future applications that require targeting individual strains in a bacterial community. </p2></div> |
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Revision as of 09:13, 28 October 2013
Operation CRISPR: Deploying precision guided tools to target unique species in a complex microbiome
1. Introducing CRISPRi to a bacterial community
1. CRISPRi utilizes gRNAs which are highly specific and customizable.
2. In principle it could be used to take advantage of unique DNA sequences to target specific bacterial species.