Team:UC Davis/Protocols

From 2013.igem.org

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<li>Once it has run, use hand held UV lamp (in the dark, wearing goggles) to identify bands. </li>
<li>Once it has run, use hand held UV lamp (in the dark, wearing goggles) to identify bands. </li>
<li>Cut out desired band with stamp pipette tip and transfer to a clean tube.  The stamp pipette tip can be left in the tube to be cleaned out with a smaller pipette tip. </li>
<li>Cut out desired band with stamp pipette tip and transfer to a clean tube.  The stamp pipette tip can be left in the tube to be cleaned out with a smaller pipette tip. </li>
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<li>Weigh gel fragments and add 200 µL Buffer NTI for every 100 mg agarose gel.</li>
<li>Weigh gel fragments and add 200 µL Buffer NTI for every 100 mg agarose gel.</li>
  Incubate sample for 5-10 min at 50º C, vortexing every 2-3 min until the gel is completely dissolved.</li>
  Incubate sample for 5-10 min at 50º C, vortexing every 2-3 min until the gel is completely dissolved.</li>
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<li>Centrifuge for 1 min at 11,000 x g. </li>
<li>Centrifuge for 1 min at 11,000 x g. </li>
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<img src="https://static.igem.org/mediawiki/2013/e/ec/UCDavis_gelExt.jpg" width=300 height=183 />
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Revision as of 01:51, 18 October 2013

Protocols