Team:UC Davis/Protocols

From 2013.igem.org

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<p>Procedure</p>
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<p>Procedure (Invitrogen Quick Plasmid Miniprep Kit)</p>
<ol>
<ol>
<li>Sediment the cells by centrifuging 1-5 mL of overnight LB-culture. Remove all medium.</li>
<li>Sediment the cells by centrifuging 1-5 mL of overnight LB-culture. Remove all medium.</li>
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<p>Procedure</p>
<p>Procedure</p>
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<li>Add equal volumes (500-700 µL) of overnight cell culture and glycerol into a cryotube, keep sterile with a flame.</li>
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<li>Add equal volumes (500-700 µL) of overnight cell culture and 80% glycerol into a cryotube, keep sterile with a flame.</li>
<li>Store at -80º C.</li>
<li>Store at -80º C.</li>
<li>When reviving a glycerol stock, keep the glycerol stock on dry ice.   
<li>When reviving a glycerol stock, keep the glycerol stock on dry ice.   

Revision as of 20:15, 19 October 2013

Protocols