Team:UNITN-Trento

From 2013.igem.org

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     <a class="silhouette" href="https://2013.igem.org/Team:UNITN-Trento/Team"><img class="photo-1" src="https://static.igem.org/mediawiki/2013/a/a5/Tn-2013-silhouette.png"><img style="display: none;" class="photo-2" src="https://static.igem.org/mediawiki/2013/2/21/Tn-2013-silhouette-2.png" /></a>
     <a class="silhouette" href="https://2013.igem.org/Team:UNITN-Trento/Team"><img class="photo-1" src="https://static.igem.org/mediawiki/2013/a/a5/Tn-2013-silhouette.png"><img style="display: none;" class="photo-2" src="https://static.igem.org/mediawiki/2013/2/21/Tn-2013-silhouette-2.png" /></a>
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         <p>Hi everybody!</p>
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        <p>Our team is proud to introduce to you <i>B. fruity</i>, a new environmentally friendly way to control climacteric and non-climacteric fruit ripening by exploiting an engineered, light regulated strain of <i>B. subtilis</i>. The system works by synthesizing ethylene or methyl salicylate (MeSA) upon photoinduction.</p>
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            <p>Hi everybody!</p>
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            <p>Our team is proud to introduce to you <i>B. fruity</i>, a new environmentally friendly way to control climacteric and non-climacteric fruit ripening by exploiting an engineered, light regulated strain of <i>B. subtilis</i>. The system works by synthesizing ethylene or methyl salicylate (MeSA) upon photoinduction.</p>
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        <p><b>Ethylene</b> is a plant hormone widely used to ripen fruit such as bananas, kiwifruit, apples, and others. However, the synthesis, handling, and storage of ethylene is expensive and dangerous. In contrast, <i>B. fruity</i> produces ethylene from inexpensive material by hijacking a metabolic intermediate, 2-oxoglutarate, from the TCA cycle and converting this metabolite to ethylene throught the activity of an ethylene forming enzyme (EFE, 2-oxoglutarate oxygenase/decarboxylase) from <i>Pseudomonas syringae</i>.</p>
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            <p><b>Ethylene</b> is a plant hormone widely used to ripen fruit such as bananas, kiwifruit, apples, and others. However, the synthesis, handling, and storage of ethylene is expensive and dangerous. In contrast, <i>B. fruity</i> produces ethylene from inexpensive material by hijacking a metabolic intermediate, 2-oxoglutarate, from the TCA cycle and converting this metabolite to ethylene throught the activity of an ethylene forming enzyme (EFE, 2-oxoglutarate oxygenase/decarboxylase) from <i>Pseudomonas syringae</i>.</p>
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        <p><i>B. fruity</i> does not just accelerate ripening, but can also slow the process down, when desired, through the incorporation of a <b>methyl salicylate</b> (MeSA) synthesis pathway. MeSA was previously shown to inhibit the ripening of kiwi and tomatoes. The explored MeSA pathway builds upone the 2006 MIT iGEM project “<i>Eau de coli</i>”.</p>
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            <p><i>B. fruity</i> does not just accelerate ripening, but can also slow the process down, when desired, through the incorporation of a <b>methyl salicylate</b> (MeSA) synthesis pathway. MeSA was previously shown to inhibit the ripening of kiwi and tomatoes. The explored MeSA pathway builds upone the 2006 MIT iGEM project “<i>Eau de coli</i>”.</p>
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        <p>As a proof of concept, we engineered <i>E. coli</i> with the above systems plus the YF1/FixJ blue light receptor device.</p>
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            <p>As a proof of concept, we engineered <i>E. coli</i> with the above systems plus the YF1/FixJ blue light receptor device.</p>
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        <p>We are hopeful that <i>B. fruity</i> will simplify the process of bringing fresh fruit from the field to the consumer.</p>
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            <p>We are hopeful that <i>B. fruity</i> will simplify the process of bringing fresh fruit from the field to the consumer.</p>
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                be fruity
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Revision as of 11:45, 20 September 2013