Team:UNITN-Trento

From 2013.igem.org

(Difference between revisions)
 
(29 intermediate revisions not shown)
Line 10: Line 10:
<!-- PAGE -->
<!-- PAGE -->
-
<div class="pre-footer">
+
<a href="http://2013.igem.org/Team:UNITN-Trento/Extra/Judging" class="news">We got gold and 'Best Poster of the World' Award!! <img class="photo" src="http://2013.igem.org/wiki/images/b/b3/Tn-2013-Gold_award.png" /></a>
 +
 
 +
<div class="pre-grid">
     <span class="silhouette">
     <span class="silhouette">
         <img class="photo-1" src="http://2013.igem.org/wiki/images/a/a5/Tn-2013-silhouette.png">
         <img class="photo-1" src="http://2013.igem.org/wiki/images/a/a5/Tn-2013-silhouette.png">
Line 17: Line 19:
     <div class="sheet">
     <div class="sheet">
         <div class="container">
         <div class="container">
-
             <p>Hi everybodya!</p>
+
             <p>Hi everybody, UniTN-Trento team is proud to introduce you <i>B. fruity</i>!</p>
              
              
-
             <p>Our team is proud to introduce to you <i>B. fruity</i>, a new environment-friendly way to control fruit ripening by exploiting an engineered light-regulated strain of <i>B. subtilis</i>. The system works by synthesizing ethylene or methyl salicylate (MeSA) upon photoinduction.</p>
+
             <p><i>B. fruity</i> envisions a new environmental friendly way to control fruit ripening by exploiting an engineered, light-regulated strain of <i>B. subtilis</i>. The system works by synthesizing ethylene or methyl salicylate (MeSA) upon photoinduction.</p>
-
             <p><b>Ethylene</b> is a plant hormone widely used to ripen fruit. However its synthesis, handling, and storage is expensive and dangerous. In contrast, <i>B. fruity</i> produces ethylene from inexpensive material by hijacking a metabolic intermediate: 2-oxoglutarate. Our system converts this metabolite to ethylene through the activity of an ethylene forming enzyme (EFE) from <i>Pseudomonas syringae</i>.</p>
+
             <p><b>Ethylene</b> is a plant hormone widely used to ripen fruit. However its synthesis, handling and storage is expensive and dangerous. In contrast, <i>B. fruity</i> produces ethylene from inexpensive material by a metabolic intermediate: 2-oxoglutarate. Our system converts this metabolite to ethylene through the activity of an ethylene forming enzyme (EFE) from <i>Pseudomonas syringae</i>.</p>
             <p><i>B. fruity</i> does not just accelerate ripening, but can also slow the process down, when desired, through the incorporation of a <b>methyl salicylate</b> (MeSA) synthesis pathway. MeSA was previously shown to inhibit the ripening of kiwifruit and tomatoes. The explored MeSA pathway builds upon the 2006 MIT iGEM project “<i>Eau de coli</i>”.</p>
             <p><i>B. fruity</i> does not just accelerate ripening, but can also slow the process down, when desired, through the incorporation of a <b>methyl salicylate</b> (MeSA) synthesis pathway. MeSA was previously shown to inhibit the ripening of kiwifruit and tomatoes. The explored MeSA pathway builds upon the 2006 MIT iGEM project “<i>Eau de coli</i>”.</p>
Line 31: Line 33:
     </div>
     </div>
</div>
</div>
 +
 +
<a href="http://2013.igem.org/Team:UNITN-Trento/Project/Ethylene#newGC" class="news-2">Check out our brand new results!!!</a>
<div class="grid">
<div class="grid">
-
    <div class="row first">
+
<div class="column first">
-
        <div class="wrap cell first">
+
<div class="cell first">
-
        <a href="http://2013.igem.org/Team:UNITN-Trento/Project/Introduction"></a>
+
<a href="http://2013.igem.org/Team:UNITN-Trento/Project/Introduction#tour"></a>
-
        </div>
+
</div>
-
        <div class="wrap cell second" id="jms-slideshow">
+
<div class="cell second">
-
            <div id="img1" class="step" style="background-image: url(http://2013.igem.org/wiki/images/6/6c/Tn-2013-home-slider-DSC_0132.jpg);" />
+
<a href="http://2013.igem.org/Team:UNITN-Trento/Project/Fruit%20ripening"></a>
-
            </div>
+
</div>
-
            <div id="img2" class="step" style="background-image: url(http://2013.igem.org/wiki/images/2/20/Tn-2013-home-slider-DSC_0021.jpg);" />
+
</div>
-
            </div>
+
<div class="column second">
-
        </div>
+
-
    </div>
+
-
    <div class="row second">
+
-
        <div class="cell first">
+
-
        <a href="http://2013.igem.org/Team:UNITN-Trento/Project/Fruit%20ripening"></a>
+
-
        </div>
+
-
        <div class="cell second">
+
-
        <a href="http://2013.igem.org/Team:UNITN-Trento/Project/Vending%20Machine"></a>
+
-
        </div>
+
-
        <div class="cell third">
+
-
        <a href="http://2013.igem.org/Team:UNITN-Trento/Extra/Fruit%20Info"></a>
+
-
        </div>
+
-
    </div>
+
-
    <div class="row third">
+
<div class="cell first">
<div class="cell first">
        <span class="tn-title">Achievements</span>
        <span class="tn-title">Achievements</span>
        <img class="plasmid" src="http://2013.igem.org/wiki/images/b/b6/Tn-2013-home-ach-Plasmids.png" />
        <img class="plasmid" src="http://2013.igem.org/wiki/images/b/b6/Tn-2013-home-ach-Plasmids.png" />
<ul class="plasmid">
<ul class="plasmid">
-
<li>Built and submitted XX New BioBricks;</li>
+
<li>Built and submitted 16 New BioBricks;</li>
-
<li>Successfully characterized YY BioBricks;</li>
+
<li>Successfully characterized 9 BioBricks;</li>
-
<li>Improved XX parts;</li>
+
<li>Improved 4 parts;</li>
</ul>
</ul>
<img class="human" src="http://2013.igem.org/wiki/images/f/f9/Tn-2013-home-ach-Human-Practice_150.png" />
<img class="human" src="http://2013.igem.org/wiki/images/f/f9/Tn-2013-home-ach-Human-Practice_150.png" />
Line 71: Line 61:
<img class="product" src="http://2013.igem.org/wiki/images/f/fb/Tn-2013-home-ach-Products.png" />
<img class="product" src="http://2013.igem.org/wiki/images/f/fb/Tn-2013-home-ach-Products.png" />
<ul class="product">
<ul class="product">
-
<li>Designed a fruit Vending Machine and an Home Edition B. fruity products;</li>
+
<li>Designed a fruit Vending Machine and a Home Edition <i>B. fruity</i> market product;</li>
</ul>
</ul>
<img class="fruit" src="http://2013.igem.org/wiki/images/3/30/Tn-2013-home-ach-Fruit.png" />
<img class="fruit" src="http://2013.igem.org/wiki/images/3/30/Tn-2013-home-ach-Fruit.png" />
<ul class="fruit">
<ul class="fruit">
-
<li>Ripened XX bananas, YY tomatoes, zz kiwis, UU plums;</li>
+
<li>Ripened 5 bananas, 5 tomatoes, 3 kiwis, 2 plums, 2 apples;</li>
<li>Eaten one banana bread, one plums crumble and one apple strudel. </li>
<li>Eaten one banana bread, one plums crumble and one apple strudel. </li>
</ul>
</ul>
-
</div>
+
</div>
-
    </div>
+
</div>
 +
<div class="column third">
 +
<div class="cell first">
 +
<a href="http://2013.igem.org/Team:UNITN-Trento/Project/Vending%20Machine"></a>
 +
</div>
 +
<div class="cell second">
 +
<a href="http://2013.igem.org/Team:UNITN-Trento/Extra/Fruit%20Info"></a>
 +
</div>
 +
</div>
</div>
</div>
<!--end content--></html>|<html>http://2013.igem.org/wiki/images/5/53/Tn-2013-headerbg-Sfondobb.png</html>|<html>http://2013.igem.org/wiki/images/d/d2/Tn-2013-headerbg-Sfondobb_or.png</html>}}
<!--end content--></html>|<html>http://2013.igem.org/wiki/images/5/53/Tn-2013-headerbg-Sfondobb.png</html>|<html>http://2013.igem.org/wiki/images/d/d2/Tn-2013-headerbg-Sfondobb_or.png</html>}}

Latest revision as of 11:32, 15 December 2013


We got gold and 'Best Poster of the World' Award!!

Hi everybody, UniTN-Trento team is proud to introduce you B. fruity!

B. fruity envisions a new environmental friendly way to control fruit ripening by exploiting an engineered, light-regulated strain of B. subtilis. The system works by synthesizing ethylene or methyl salicylate (MeSA) upon photoinduction.

Ethylene is a plant hormone widely used to ripen fruit. However its synthesis, handling and storage is expensive and dangerous. In contrast, B. fruity produces ethylene from inexpensive material by a metabolic intermediate: 2-oxoglutarate. Our system converts this metabolite to ethylene through the activity of an ethylene forming enzyme (EFE) from Pseudomonas syringae.

B. fruity does not just accelerate ripening, but can also slow the process down, when desired, through the incorporation of a methyl salicylate (MeSA) synthesis pathway. MeSA was previously shown to inhibit the ripening of kiwifruit and tomatoes. The explored MeSA pathway builds upon the 2006 MIT iGEM project “Eau de coli”.

As a proof of concept, we engineered E. coli with the above system plus the YF1/FixJ blue light receptor device.

We are hopeful that B. fruity will simplify the process of bringing fresh fruit from the field to the consumer.

Check out our brand new results!!!
Achievements
  • Built and submitted 16 New BioBricks;
  • Successfully characterized 9 BioBricks;
  • Improved 4 parts;
  • Hosted a high school student in the lab for the summer;
  • Asked the opinion of local fruit consumers on our project;
  • Designed a fruit Vending Machine and a Home Edition B. fruity market product;
  • Ripened 5 bananas, 5 tomatoes, 3 kiwis, 2 plums, 2 apples;
  • Eaten one banana bread, one plums crumble and one apple strudel.