Team:UNITN-Trento/Notebook/Labposts/06/15

From 2013.igem.org

(Difference between revisions)
(Created page with "{ "date" : "2013-06-07", "author" : "bruno", "title" : "Preparation of stock solution Ampicillin and Chloramphenicol", "content" : "<html>I have prepared the stock solution, ...")
 
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{
{
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"date" : "2013-06-07",
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"date" : "2013-06-11",
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"author" : "bruno",
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"author" : "thomas-michele",
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"title" : "Preparation of stock solution Ampicillin and Chloramphenicol",
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"title" : "Transformation Test!",
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"content" : "<html>I have prepared the stock solution, 10 ml, for the antibiotic resistance, specifically I have prepared a stock for Ampicillin and Chloramphenicol respectively 50 mg/ml and 34 mg/ml. I have prepared starting from the dust. <br/>Particular care to use Distilled water for Ampicillin and Ethanol for Chloramphenicol. </html>",
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"content" : "To understand if our Neb10<html>&beta;</html> cells and all our reagents for transformation worked well, we transformed 200 ul of cells with 1 ul of pSB1C3 with RFP, previously purificated. As you can see from the picture we've done a nice job!! {{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plate Image|<html><img id=\"post_img\" src=\"https://static.igem.org/mediawiki/2013/3/3c/Tn-20130611-plate.jpg\" width=\"500px\"/></html>}}",
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"tags" : "mix"
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"tags" : "RFP"
}
}

Latest revision as of 07:42, 3 October 2013

{ "date" : "2013-06-11", "author" : "thomas-michele", "title" : "Transformation Test!", "content" : "To understand if our Neb10β cells and all our reagents for transformation worked well, we transformed 200 ul of cells with 1 ul of pSB1C3 with RFP, previously purificated. As you can see from the picture we've done a nice job!!

", "tags" : "RFP" }