Team:UNITN-Trento/Notebook/Labposts/06/23

From 2013.igem.org

(Difference between revisions)
 
Line 1: Line 1:
{
{
"date" : "2013-06-13",
"date" : "2013-06-13",
-
"author" : "bruno-fabio",
+
"author" : "gabriele",
-
"title" : "All day...all night...antibiotic plates",
+
"title" : "Ligation results: let's screen it!",
-
"content" : "<html>We wanted to do a trasformation but...we don't have the plates. So, at a good pace, we started to make plates with ampicillin and chloramphenicol.</html>",
+
"content" : "<html>Both the 1:2 ligation plates have number of colonies comparable with their control:<br/><ul><li>SAMsynthetase+RFP (1:2) = too many to count.</li><li>SAMsynthetase+RFP (ctrl) = too many to count.</li><li>SAMsynthetase (1:2) = 11 colonies.</li><li>SAMsynthetase (ctrl) = 12 colonies.</li></ul><br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|SAMsynthetase+pSB1C3[RFP] (1:2) plate|<html><center><img src=\"https://static.igem.org/mediawiki/2013/8/8e/Tn-20130613-SAM_RFP_1_2.JPG\" width=\"300px\" /></center></html>}}<html></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|SAMsynthetase+pSB1C3[RFP] (ctrl) plate|<html><center><img src=\"https://static.igem.org/mediawiki/2013/c/ce/Tn-20130613-SAM_RFP_ctrl.JPG\" width=\"300px\" /></center></html>}}<html></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|SAMsynthetase+pSB1C3 (1:2) plate|<html><center><img src=\"https://static.igem.org/mediawiki/2013/f/f9/Tn-20130613-SAM_1_2.JPG\" width=\"300px\" /></center></html>}}<html></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|SAMsynthetase+pSB1C3 (ctrl) plate|<html><center><img src=\"https://static.igem.org/mediawiki/2013/b/bd/Tn-20130613-SAM_ctrl.JPG\" width=\"300px\" /></center></html>}}<html><br/>Performed 5 inocula for each of the two 1:2 plates, in 5ml LB with 5&micro;l CM. Tomorrow miniprep, quantification, digestion and gel to control what happened.</html>",
-
"tags" : "mix"
+
"tags" : "SAMsynthetase"
}
}

Latest revision as of 07:44, 3 October 2013

{ "date" : "2013-06-13", "author" : "gabriele", "title" : "Ligation results: let's screen it!", "content" : "Both the 1:2 ligation plates have number of colonies comparable with their control:

  • SAMsynthetase+RFP (1:2) = too many to count.
  • SAMsynthetase+RFP (ctrl) = too many to count.
  • SAMsynthetase (1:2) = 11 colonies.
  • SAMsynthetase (ctrl) = 12 colonies.

SAMsynthetase+pSB1C3[RFP] (1:2) plate
SAMsynthetase+pSB1C3[RFP] (ctrl) plate
SAMsynthetase+pSB1C3 (1:2) plate
SAMsynthetase+pSB1C3 (ctrl) plate

Performed 5 inocula for each of the two 1:2 plates, in 5ml LB with 5µl CM. Tomorrow miniprep, quantification, digestion and gel to control what happened.", "tags" : "SAMsynthetase" }

Retrieved from "http://2013.igem.org/Team:UNITN-Trento/Notebook/Labposts/06/23"