From 2013.igem.org
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| - | { | + | {"date" : "2013-07-02","author" : "caterina-michele","title" : "Incredible: E.coli could growth!","content" : " Today was a nice day! Finally Michele’s plate were positive (no colonies on the control and lot of them on the others!) So at the end of the day we could do the inocula. Moreover Michele finished the reaction of ligation with the plasmid pSB1C3 linearized (digested the day previous by Caterina (link post)) and J45319 and transformed it in 200 ul of NebB10. At the end of the day Caterina plate them. Everything is working. Hoping that Mesa will be detectable! ","tags" : "PchBA-BMST1-araC-pBAD"} |
| - | "date" : "2013-07-02",
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| - | "author" : "fabio-viola",
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| - | "title" : "BACILLUS SUBTILIS COMES BACK TO LIFE!",
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| - | "content" : "<html> in order to propagate B. subtilis from the pellet that we purchased (strain ind- tyr+ B.subtilis ATCC 23857) , first we needed to obtain the perfect medium for its growth. We prepared Nutrient Broth for liquid cultures and Nutrient Broth + Agar for plates, following ATCC PROTOCOLS. So we decided to have them in both a STARCH-added version and a version without starch.<br>Nutrient agar (100 ml) : 0,8 g nutrient broth: 1,5g Agar; water to 100 ml.<br>Nutrient agar + starch (100 ml) : 0,8 g nutrient broth: 1,5g Agar; 2ml starch from potato, water up to 100 ml.<br>Nutrient broth (250 ml): 2 g nutrient broth; 250 ml water.<br>Nutrient broth + starch (250 ml): 2 g nutrient broth; 5 ml starch; water up to 250 ml; <br>To propagate di original bacillus pellet, we resuspended it in 1 ml of nutrient broth+starch and put this milliliter in 5 ml of nutrient broth+starch for a final 6 ml mother liquid culture. Then we made several other liquid cultures with different bacteria concentrations from the Mother (for each concentration we made both starch and non-starch liquid cultures). We put all in a shaker at 26 degrees. We plated also the same concentrations in several plates with both Nutrient agar + starch and Nutrient agar alone, and put them at 26. Just out of curiosity we decided to put some plates and some liquid cultures at 37 degrees.</html>",
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| - | "tags" : " Bacillus subtilis "
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| - | } | + | |
Latest revision as of 10:33, 3 October 2013
{"date" : "2013-07-02","author" : "caterina-michele","title" : "Incredible: E.coli could growth!","content" : " Today was a nice day! Finally Michele’s plate were positive (no colonies on the control and lot of them on the others!) So at the end of the day we could do the inocula. Moreover Michele finished the reaction of ligation with the plasmid pSB1C3 linearized (digested the day previous by Caterina (link post)) and J45319 and transformed it in 200 ul of NebB10. At the end of the day Caterina plate them. Everything is working. Hoping that Mesa will be detectable! ","tags" : "PchBA-BMST1-araC-pBAD"}
"
