Team:UNITN-Trento/Notebook/Labposts/07/12

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{"date":"2013-07-03","author":"viola","title":"<html>cloning of the terminators with EFE</html>","content":"<html> <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-02-viola\">Yesterday's ligation</a> seemed to work and and this evening i'll make some inocula... </html>","tags":"EFE"}
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"date" : "2013-07-10",
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"author" : "fabio",
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"title" : " ripenator v1.0 ",
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"content" : "<html> this morning, Bruno is not available, so I will take care of the ripenator. I have to replace all the cultures with new LB and new bacteria : I’m gonna put 300 ml of LB in each beutas, then connect immediately a beuta with LB and 3ml of not-induced bacteria to the control banana!! After that, I will dissolve 78 ul of MESA in the second beuta and connect to the blocked banana! As far as the ethylene sample is concerned, I have to put 3 ml of bacteria in the last beuta, wait for the OD lecture to reach the value of 0,5 and then induce the culture with Arabinose to produce Ethylene! Finally I can connect it with the mature banana. </html>",
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"tags" : " ripenator-mesa-ethylene "
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}
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Latest revision as of 10:34, 3 October 2013

{"date":"2013-07-03","author":"viola","title":"cloning of the terminators with EFE","content":" Yesterday's ligation seemed to work and and this evening i'll make some inocula... ","tags":"EFE"}

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