Team:UNITN-Trento/Notebook/Labposts/07/21

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Revision as of 08:28, 3 October 2013

{ "date" : "2013-07-10", "author" : "emil", "title" : " Purification of Inocula(09/7): the final countdown", "content" : "I (yes I'm egocentric) did the miniprep(wath a new thing!) of the inocula(I didn't purified the 1:2 due to their despicable red color) previously done(the last I hope) following(is always the same but I have to write it anyway)the miniprep protocol(OOOH!that's incredible!).These are(obviously)the results of the quantification:

results

tr>tr>

Sample	Quantities
BBa_K823026+BBa_E0840(1:1)	421.3ng/µl
BBa_K823026+BBa_E0840(1:1)	438.6ng/µl
BBa_K823026+BBa_E0840(1:3)	356.7ng/µl
BBa_K823026+BBa_E0840(1:3)	415.2ng/µl
BBa_K823024+BBa_E0840	401.2ng/µl
BBa_K823024+BBa_E0840	365ng/µl

Afterwards I screened them(800 ng for each sample) following the digestion protocol so I have obtained these results:

gel order

tr>tr>

Sample	Well
ladder	1
BBa_K823026+BBa_E0840(1:1)	2
BBa_K823026+BBa_E0840(1:1)	3
BBa_K823026+BBa_E0840(1:3)	4
BBa_K823026+BBa_E0840(1:3)	5
BBa_K823024+BBa_E0840	6
BBa_K823024+BBa_E0840	7

image

As we can see in the last 4 samples the lower band is clearly at 1000 bp then the GFP is probably present, the succesfull samples were sent to be sequenced!", "tags" : "pSpac-PXyl-GFP" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-07-24",
+	"date" : "2013-07-10",
-	"author" : "fabio-bruno",
+	"author" : "emil",
-	"title" : " the blue ligation: the never ending story !",
+	"title" : " Purification of Inocula(09/7): the final countdown",
-	"content" : "<html>Today I inoculated 6 colonies of the plated ligation ( 3 from the 1:1 plate  and 2 from the 1:3 plate) <br>I also made the Onetaq Pcr for all the light parts ( blue device,the b sample from the first set; red device, from the third set; and red promoter, from the third set). <br>After that I screened them all and found out that only the blue part was confirmed: up to now we only have the  blue parts confirmed!! Red parts , it seems to me that you’re in hot waters !! <br></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Immagine gel|<html><center><img src=\"https://static.igem.org/mediawiki/2013/7/7f/Tn-2013_Daa.jpg\" width=\"450px\" /></center></html>}}<html></html>",
+	"content" : "<html>I (yes I'm egocentric) did the miniprep(wath a new thing!) of the inocula(I didn't purified the 1:2 due to their despicable red color) previously done(the last I hope) following(is always the same but I have to write it anyway)the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#miniprep\">miniprep protocol</a>(OOOH!that's incredible!).These are(obviously)the results of the quantification:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|results|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Quantities</th></tr><tr><td>BBa_K823026+BBa_E0840(1:1) </td><td> 421.3ng/&micro;l</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) </td><td> 438.6ng/&micro;l</td></tr>tr><td>BBa_K823026+BBa_E0840(1:3) </td><td> 356.7ng/&micro;l</td></tr><tr><td>BBa_K823026+BBa_E0840(1:3) </td><td> 415.2ng/&micro;l</td></tr><tr><td>BBa_K823024+BBa_E0840 </td><td> 401.2ng/&micro;l</td></tr>tr><td>BBa_K823024+BBa_E0840 </td><td> 365ng/&micro;l</td></tr></table></center></html>}}<html>Afterwards I screened them(800 ng for each sample) following the <a href =\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion\">digestion protocol</a> so I have obtained these results:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel order|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Well</th></tr>                <tr><td>ladder </td><td>1</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) </td><td>2</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) </td><td>3</td></tr>tr><td>BBa_K823026+BBa_E0840(1:3) </td><td>4</td></tr><tr><td>BBa_K823026+BBa_E0840(1:3) </td><td>5</td></tr><tr><td>BBa_K823024+BBa_E0840 </td><td>6</td></tr>tr><td>BBa_K823024+BBa_E0840 </td><td>7</td></tr></table></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/1/15/Tn-2013_DSC0161.JPG\" width=\"450px\"></html>}}<html>As we can see  in the last 4 samples the lower band is clearly at 1000 bp then the GFP is probably present, the  succesfull samples were sent to be sequenced!</html>",
-	"tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"
+	"tags" : "pSpac-PXyl-GFP"
 }