Team:UNITN-Trento/Notebook/Labposts/07/21

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Latest revision as of 10:37, 3 October 2013

{"date" : "2013-07-08","author" : "thomas","title" : "Ethylene production kinetic assay (second trial)","content" : "This morning I repeated the experiment done the last thursday. This time however, I used the same vial used during the ethylene detection experiment. This time the experiment worked as expected and I successfully plotted the data.

Plot

A sample of the same stock culture was kept inthermoshaker. As expected an higher value of ethylene was detected (see the green point) since it was subjected to only one measure (thus having no gas loss due to repeated measures). ","tags" : "EFE"}

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+{"date" : "2013-07-08","author" : "thomas","title" : "Ethylene production kinetic assay (second trial)","content" : "<html>This morning I repeated <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-04-thomas\">the experiment</a> done the last thursday. This time however, I used the same vial used during the <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-06-28-thomas\">ethylene detection experiment</a>. This time the experiment worked as expected and I successfully plotted the data.  <br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plot|<html><center><img src=\"https://static.igem.org/mediawiki/2013/5/50/Tn-2013_kinetic_plot-1.png\" style =\"width: 450px\"></center></html>}}<html>A sample of the same stock culture was kept inthermoshaker. As expected an higher value of ethylene was detected (see the green point) since it was subjected to only one measure (thus having no gas loss due to repeated measures). </html>","tags" : "EFE"}
-	"date" : "2013-07-10",
-	"author" : "emil",
-	"title" : " Purification of Inocula(09/7): the final countdown",
-	"content" : "<html>I (yes I'm egocentric) did the miniprep(wath a new thing!) of the inocula(I didn't purified the 1:2 due to their despicable red color) previously done(the last I hope) following(is always the same but I have to write it anyway)the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#miniprep\">miniprep protocol</a>(OOOH!that's incredible!).These are(obviously)the results of the quantification:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|results|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Quantities</th></tr><tr><td>BBa_K823026+BBa_E0840(1:1) </td><td> 421.3ng/&micro;l</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) </td><td> 438.6ng/&micro;l</td></tr>tr><td>BBa_K823026+BBa_E0840(1:3) </td><td> 356.7ng/&micro;l</td></tr><tr><td>BBa_K823026+BBa_E0840(1:3) </td><td> 415.2ng/&micro;l</td></tr><tr><td>BBa_K823024+BBa_E0840 </td><td> 401.2ng/&micro;l</td></tr>tr><td>BBa_K823024+BBa_E0840 </td><td> 365ng/&micro;l</td></tr></table></center></html>}}<html>Afterwards I screened them(800 ng for each sample) following the <a href =\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion\">digestion protocol</a> so I have obtained these results:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel order|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Well</th></tr>                <tr><td>ladder </td><td>1</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) </td><td>2</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) </td><td>3</td></tr>tr><td>BBa_K823026+BBa_E0840(1:3) </td><td>4</td></tr><tr><td>BBa_K823026+BBa_E0840(1:3) </td><td>5</td></tr><tr><td>BBa_K823024+BBa_E0840 </td><td>6</td></tr>tr><td>BBa_K823024+BBa_E0840 </td><td>7</td></tr></table></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/1/15/Tn-2013_DSC0161.JPG\" width=\"450px\"></html>}}<html>As we can see  in the last 4 samples the lower band is clearly at 1000 bp then the GFP is probably present, the  succesfull samples were sent to be sequenced!</html>",
-	"tags" : "pSpac-PXyl-GFP"
-}