Team:UNITN-Trento/Notebook/Labposts/07/21

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(Created page with "{ "date" : "2013-07-24", "author" : "fabio-bruno", "title" : " the blue ligation: the never ending story !", "content" : "<html>Today I inoculated 6 colonies of the plated li...")
 
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{"date" : "2013-07-08","author" : "thomas","title" : "Ethylene production kinetic assay (second trial)","content" : "<html>This morning I repeated <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-04-thomas\">the experiment</a> done the last thursday. This time however, I used the same vial used during the <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-06-28-thomas\">ethylene detection experiment</a>. This time the experiment worked as expected and I successfully plotted the data. <br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plot|<html><center><img src=\"https://static.igem.org/mediawiki/2013/5/50/Tn-2013_kinetic_plot-1.png\" style =\"width: 450px\"></center></html>}}<html>A sample of the same stock culture was kept inthermoshaker. As expected an higher value of ethylene was detected (see the green point) since it was subjected to only one measure (thus having no gas loss due to repeated measures). </html>","tags" : "EFE"}
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"date" : "2013-07-24",
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"author" : "fabio-bruno",
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"title" : " the blue ligation: the never ending story !",
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"content" : "<html>Today I inoculated 6 colonies of the plated ligation ( 3 from the 1:1 plate  and 2 from the 1:3 plate) <br>I also made the Onetaq Pcr for all the light parts ( blue device,the b sample from the first set; red device, from the third set; and red promoter, from the third set). <br>After that I screened them all and found out that only the blue part was confirmed: up to now we only have the  blue parts confirmed!! Red parts , it seems to me that you’re in hot waters !! <br></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Immagine gel|<html><center><img src=\"https://static.igem.org/mediawiki/2013/7/7f/Tn-2013_Daa.jpg\" width=\"450px\" /></center></html>}}<html></html>",
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"tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"
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}
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Latest revision as of 10:37, 3 October 2013

{"date" : "2013-07-08","author" : "thomas","title" : "Ethylene production kinetic assay (second trial)","content" : "This morning I repeated the experiment done the last thursday. This time however, I used the same vial used during the ethylene detection experiment. This time the experiment worked as expected and I successfully plotted the data.

A sample of the same stock culture was kept inthermoshaker. As expected an higher value of ethylene was detected (see the green point) since it was subjected to only one measure (thus having no gas loss due to repeated measures). ","tags" : "EFE"}