Team:UNITN-Trento/Notebook/Labposts/07/45

From 2013.igem.org

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{"date" : "2013-07-20","author" : "fabio-bruno","title" : " blue and red light sensors!!","content" : "<html> this morning I made some minipreps from last night inocula ( third set) instead Bruno perform the same protocol on the second set of inocula. <br>Second set yields are: <br>006a : 289 <br>006b : 409.3<br>016° : 188.8<br>016b :266.6<br>030a : 388.6<br>030b : 270<br>082a : 433.0<br>082b : 568<br>Third set yiealds are: <br>006: 321 ng/ul<br>016: 440.9 ng/ul<br>R0082: 441,5 ng/ul<br>030: 199,9 ng/ul<br>These ones haven’t been screened so far. <br>Meanwhile, we extracted a latter incredible part: k952003, which contains the blue device, the blue promoter and a fluorescent reporter.</html>","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"}
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"date" : "2013-07-22",
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"author" : "thomas",
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"title" : "Inocula of AraC-pBAD + EFE + Venus",
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"content" : "<html>This is the part four of <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-18-thomas-michele\">this experiment</a>. I proceeded preparing 5 inocula from the 1:1 and 1:4 ligation product Plates. The inocula were grown overnight at 37&deg;C in thermoshaker. </html>",
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"tags" : "EFE-Venus"
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}
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Latest revision as of 10:43, 3 October 2013

{"date" : "2013-07-20","author" : "fabio-bruno","title" : " blue and red light sensors!!","content" : " this morning I made some minipreps from last night inocula ( third set) instead Bruno perform the same protocol on the second set of inocula.
Second set yields are:
006a : 289
006b : 409.3
016° : 188.8
016b :266.6
030a : 388.6
030b : 270
082a : 433.0
082b : 568
Third set yiealds are:
006: 321 ng/ul
016: 440.9 ng/ul
R0082: 441,5 ng/ul
030: 199,9 ng/ul
These ones haven’t been screened so far.
Meanwhile, we extracted a latter incredible part: k952003, which contains the blue device, the blue promoter and a fluorescent reporter.","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"}