Team:UNITN-Trento/Notebook/Labposts/07/47

From 2013.igem.org

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{"date" : "2013-07-21","author" : "fabio-bruno","title" : " blue and red light sensors!!","content" : "<html> today we decided to get our parts sequenced, so I prepared sequencing samples and tomorrow they will be sent to a sequencing company. <br>We also inoculate the new part: k952003. From now on Bruno and I will take our own paths!!</html>","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR-YF1_FixJ_PfixK2_amilGFP"}
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"date" : "2013-07-23",
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"author" : "fabio",
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"title" : " the blue ligation: the never ending story !",
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"content" : "<html> this morning I added Sap to the 016 digestion and dPnl to the other two, then I purified them and quantified'em: <br>O16: 29,8 ng/ul <br>006: 16,2 ng/ul <br>R0010: 6,1 ng/ul <br>Then I started the ligation following the ligation protocol: at the end of it, I transformed 10ul of the ligation in 200 Neb10b and plated.Meanwhile Bruno miniprepped k952003 inocula and the screening was successful!!</html>",
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"tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR- YF1_FixJ_PfixK2_amilGFP "
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Latest revision as of 10:43, 3 October 2013

{"date" : "2013-07-21","author" : "fabio-bruno","title" : " blue and red light sensors!!","content" : " today we decided to get our parts sequenced, so I prepared sequencing samples and tomorrow they will be sent to a sequencing company.
We also inoculate the new part: k952003. From now on Bruno and I will take our own paths!!","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR-YF1_FixJ_PfixK2_amilGFP"}