Team:UNITN-Trento/Notebook/Labposts/07/60

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(Difference between revisions)
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{
{
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"date" : "2013-07-15",
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"date" : "2013-07-29",
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"author" : "thomas",
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"author" : "fabio",
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"title" : "Ethylene production kinetic assay (third and last trial)",
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"title" : " the blue ligation: the never ending story !",
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"content" : "<html>Since I was not satisfied with the curve obtained during the <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-08-thomas\">last experiment</a>, I decided to redo the experiment, this time starting from a culture with O.D.600 = 0,8 and registering a measurment every 45 min.  <br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plot|<html><center><img src=\"https://static.igem.org/mediawiki/2013/0/00/Tn-2013_kinetic_EFE_plot-2.png\" style =\"width: 450px\"></center></html>}}<html>Interestingly, inducing with arabinose when the sample has reached OD600 0.8 causes an increase of more than two fold in the production of ethylene.</html>",
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"content" : "<html> here we go again!! Today I miniprepped the third ligation inocula and the screening as asual wasn’t successful!! I’m kinda bummed!! At list I have my fourth and last ligation: I made 6 inocula from the plate 1:1. Will see tomorrow!</html>",
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"tags" : "EFE"
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"tags" : " YF1_FixJ - FixK2"
}
}

Revision as of 08:45, 3 October 2013

{ "date" : "2013-07-29", "author" : "fabio", "title" : " the blue ligation: the never ending story !", "content" : " here we go again!! Today I miniprepped the third ligation inocula and the screening as asual wasn’t successful!! I’m kinda bummed!! At list I have my fourth and last ligation: I made 6 inocula from the plate 1:1. Will see tomorrow!", "tags" : " YF1_FixJ - FixK2" }

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