Team:UNITN-Trento/Notebook/Labposts/07/74

From 2013.igem.org

(Difference between revisions)
(Created page with "{ "date":"2013-07-01", "author":"viola", "title":"<html>PCR of the double terminator BBa_B0015</html>", "content":"<html>Today i performed two PCR of the terminators both from th...")
Line 3: Line 3:
"author":"viola",
"author":"viola",
"title":"<html>PCR of the double terminator BBa_B0015</html>",
"title":"<html>PCR of the double terminator BBa_B0015</html>",
-
"content":"<html>Today i performed two PCR of the terminators both from the template in pSB1AK3 and from pSB1C£ following this quantities: <table> <tr> <th></th> <th>B0015 in pSB1AK3 <br/> 219,4 ng/ µl </th> <th>B0015 in pSB1C3 <br/> 413,3 ng/ µl </th></tr> <tr> <td>H20</td> <td>33.3 µl </td> <td>33.3 µl</td> </tr> <tr> <td>DNA</td> <td>0.5 µl</td> <td>0.5 µl</td> </tr> <tr> <td>DNTPs</td> <td>1 µl</td> <td>1 µl</td> </tr> <tr> <td>pref. FW</td> <td>2.5 µl</td> <td>2.5 µl</td> </tr> <tr> <td>suff. RV</td> <td>2.5 µl</td> <td>2.5 µl</td> </tr> <tr> <td>BUFFER</td> <td>10 µl</td> <td>10 µl</td> </tr> <tr> <td>Phusion pol.</td> <td>0.5 µl</td> <td>0.5 µl</td> </tr> </table> </html>}}</html>",
+
"content":"<html>Today i performed two PCR of the terminators both from the template in pSB1AK3 and from pSB1C£ following this quantities: <table> <tr> <th></th> <th>B0015 in pSB1AK3 <br/> 219,4 ng/ µl </th> <th>B0015 in pSB1C3 <br/> 413,3 ng/ µl </th></tr> <tr> <td>H20</td> <td>33.3 µl </td> <td>33.3 µl</td> </tr> <tr> <td>DNA</td> <td>0.5 µl</td> <td>0.5 µl</td> </tr> <tr> <td>DNTPs</td> <td>1 µl</td> <td>1 µl</td> </tr> <tr> <td>pref. FW</td> <td>2.5 µl</td> <td>2.5 µl</td> </tr> <tr> <td>suff. RV</td> <td>2.5 µl</td> <td>2.5 µl</td> </tr> <tr> <td>BUFFER</td> <td>10 µl</td> <td>10 µl</td> </tr> <tr> <td>Phusion pol.</td> <td>0.5 µl</td> <td>0.5 µl</td> </tr> </table> </html>}}</html>" I loaded the samples without dye and with 30% glycerol and this is the picture of the gel :</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/3/37/Tn-2013_Corsa20minPCR_B0015.jpg\" width=\"450px\" /></center></html>}}<html> on the left of the central 100bp ladder ther is B0015 in psb1ak3 and on the right there is B0015 in psb1c3.,
"tags":"EFE"
"tags":"EFE"
}
}

Revision as of 11:47, 29 September 2013

{ "date":"2013-07-01", "author":"viola", "title":"PCR of the double terminator BBa_B0015", "content":"Today i performed two PCR of the terminators both from the template in pSB1AK3 and from pSB1C£ following this quantities:

B0015 in pSB1AK3
219,4 ng/ µl 		B0015 in pSB1C3
413,3 ng/ µl
H20 33.3 µl 33.3 µl
DNA 0.5 µl 0.5 µl
DNTPs 1 µl 1 µl
pref. FW 2.5 µl 2.5 µl
suff. RV 2.5 µl 2.5 µl
BUFFER 10 µl 10 µl
Phusion pol. 0.5 µl 0.5 µl
}}</html>" I loaded the samples without dye and with 30% glycerol and this is the picture of the gel :</html>
Gel image
on the left of the central 100bp ladder ther is B0015 in psb1ak3 and on the right there is B0015 in psb1c3., "tags":"EFE" }

Retrieved from "http://2013.igem.org/Team:UNITN-Trento/Notebook/Labposts/07/74"