Team:UNITN-Trento/Notebook/Labposts/07/75

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{ "date":"2013-07-02", "author":"viola", "title":"EFE+B0015", "content":"today i digested B015 in pSB1C3 with the enzymes E and X and EFE in pUC57 with E and S. I started a short cloning protocol with this ligation quantities:

LIGATION CTRL 1:1 1:2 1:4
BUFFER T4 2 µl 2 µl 2 µl 2 µl
PLASMID (B0015) 2.5 µl 2.5 µl 2.5 µl 2.5 µl
INSEERT (EFE) 0 µl 1.28 µl 2.56 µl 5.12 µl
LIGASE T4 1 µl 1 µl 1 µl 1 µl
H2O 14.5 µl 13.2 µl 11.9 µl 10.6 µl
", "tags":"EFE" }