Team:UNITN-Trento/Notebook/Labposts/07/78
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(Created page with "{ "date":"2013-07-06", "author":"viola", "title":"<html>cloning of EFE in pSPAC and pXyl vectors</html>", "content":"<html> Today i start the cloning to insert the ethylene formi...")
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(Created page with "{ "date":"2013-07-06", "author":"viola", "title":"<html>cloning of EFE in pSPAC and pXyl vectors</html>", "content":"<html> Today i start the cloning to insert the ethylene formi...")
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Revision as of 13:45, 29 September 2013
{ "date":"2013-07-06", "author":"viola", "title":"cloning of EFE in pSPAC and pXyl vectors", "content":" Today i start the cloning to insert the ethylene forming enzyme in both the bacillus plasmids. To make this i digested the vectors and also EFE+B0015 in pSB1C3 with EcorI and PstI. {{:Team:UNITN-Trento/Templates/Styles/Spoiler|LIGATION TABLE 1|
LIGATION | EFE+B0015 in pSPAC | EFE+B0015 in pXYL |
---|---|---|
PLASMID CONC. | 20 µl | 20 µl |
PLASMID LENGHT | 7082 BP | 3594 BP |
INSERT CONCENTRATION | 20 µl | 20 µl |
INSERT LENGHT | 1251 BP | 1251 BP |
PLASMID USED | 50 ng/µl | 50 ng/µl |
REACTION VOLUME | 20 µl | 20 µl |
BUFFER | 10 µl | 10 µl |
LIGATION TABLE PSPAC
EFE IN PSPAC | CTRL | 1:1 | 1:2 | 1:4 |
---|---|---|---|---|
INSERT (µl) | 0 | 0.44 | 0.88 | 1.76 |
WATER (µl) | 14.5 | 14.06 | 13.62 | 12.74 |
LIGASE (µl) | 1 | 1 | 1 | 1 |
PLASMID (µl) | 2.5 | 2.5 | 2.5 | 2.5 |
BUFFER (µl) | 2 | 2 | 2 | 2 |
LIGATION TABLE PXYL
|BACILLUS}} |BACILLUS}} </html>",
"tags":"BACILLUS"
}
EFE IN PXYL | CTRL | 1:1 | 1:2 | 1:4 |
---|---|---|---|---|
INSERT (µl) | 0 | 0.87 | 1.74 | 3.48 |
WATER (µl) | 14.5 | 13.63 | 12.76 | 11.02 |
LIGASE (µl) | 1 | 1 | 1 | 1 |
PLASMID (µl) | 2.5 | 2.5 | 2.5 | 2.5 |
BUFFER (µl) | 2 | 2 | 2 | 2 |